相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-1502R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-CXCL10/IP10/FITC
- 抗体名:
Anti-CXCL10/IP10/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat,
- 适应物种:
Human, Mouse, Rat,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-CXCL10/IP10/FITC |
| 中文名称 | FITC标记的CXCL10趋化因子10/干扰素诱导蛋白10抗体 |
| 别 名 | 10 kDa interferon gamma induced protein; C7; Chemokine (C X C motif) ligand 10; Chemokine CXC motif ligand 10; Crg 2; CRG2; CXCL 10; CXCL10; Gamma IP10; gIP 10; GIP10; IFI 10; IFI10; INP 10; INP10; Interferon activated gene 10; Interferon gamma induced cell line; Interferon inducible cytokine IP 10; MOB1; SCYB 10; SCYB10; Small inducible cytokine B10; Small inducible cytokine B10 precursor; Small inducible cytokine subfamily B (Cys X Cys) member 10; Gamma-IP-10. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 10kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CXCL10 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. Function: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Subcellular Location: Secreted. Post-translational modifications: CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. Similarity: Belongs to the intercrine alpha (chemokine CxC) family. Database links: Entrez Gene: 3627 Human Entrez Gene: 15945 Mouse Entrez Gene: 245920 Rat Omim: 147310 Human SwissProt: P02778 Human SwissProt: P17515 Mouse SwissProt: P48973 Rat Unigene: 632586 Human Unigene: 877 Mouse Unigene: 10584 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 取消WB; 2017.4.20; 姬; |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人γ干扰素诱导蛋白16/p16(IFI16/p16)酶联免疫分析(ELISA)
板,制成固相抗体,往包被单抗的微孔中依次加入γ干扰素诱导蛋白 16/p16 ( IFI16/p16) ,再与 HRP 标记的γ干扰素诱导蛋白 16/p16 ( IFI16/p16) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的γ干扰素诱导蛋白 16/p16 ( IFI16/p16) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算
抗大鼠波形蛋白单克隆抗体,l:200稀释度),4℃过夜或37℃孵育30分钟,BS漂洗3次,每次5分钟; (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1:100),37℃避光孵育30分钟,PBS漂洗3次,每次5分钟; (8)Hoechst 333442复染细胞核15~20分钟(不是必需步骤); (9)甘油磷酸缓冲液封片,荧光显微镜下镜观察; (10)结果分析:波形蛋白免疫反应阳性细胞胞质呈现绿色荧光,如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
3和CCR3/4,因而可吸引不同的趋化因子,前者包括干扰素诱导蛋白(1P-10)、干扰素诱导性T细胞趋化蛋白(1TAC),干扰素诱生单核因子(MIG);而后者包括嗜酸粒细胞趋化蛋白Eotaxin等。现知细胞因子IL-4促进Th2分化,第十章中还将提到IL-4能促进IgE类别转换,而且IL-4(以及IL-13)还可诱导多种细胞包括单核细胞和DC产生趋化因子。结果是,这些趋化因子受体可专一性地吸引表达CCR3和CCR4的Th2/Tc2细胞,以及表达CCR3和IgE受体的嗜碱粒细胞与肥大细胞。这样,细胞
技术资料暂无技术资料 索取技术资料









