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- 文献和实验
- 技术资料
- 英文名:
Antibody Stabilizer TRIS 125 mL
- 库存:
4-6W
- 保存条件:
低温
- 供应商:
上海研卉
- 规格:
125 mL
Ready-to-use
order number 130 125
Antibody Stabilizer PBS 125 mL
Antibody Stabilizer
Stabilizer for long-term storage of proteins or antibodies at 2-8°C
Antibody Stabilizer conserves the structure of proteins and antibodies preventing them from losing functionality due to storage.
Just dissolve proteins or antibodies with Antibody Stabilizer and store in the refrigerator.
Choose from TRIS- or PBS-based Stabilizer.
Allowing you to select the buffer base, which is optimal for your assay.
For research use only, not for diagnostic use
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文献和实验the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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