- 询价
- gelatins
- JLC221591
- 上海
- 2025年07月12日
- Rabbit
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-ACTN1 antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC221591
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ACTN1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ACTN1
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ACTN1
Full name actinin, alpha 1
Synonyms BDPLT15
Swissprot P12814
Target Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 15-50 Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 15-50
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221591(ACTN1 Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml221591(ACTN1 Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:103/106 kDa
Positive control:231，A549 and Hela cell，Human fetal muscle tissue lysates
Recommended dilution: 500-2000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-4: 231，A549 and Hela cell，Human fetal muscle tissue lysates
Primary antibody: ml221591(ACTN1 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-5000
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
