- 询价
- gelatins
- JLC221156
- 上海
- 2025年07月14日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-PRCP antibody
- 宿主：
Rabbit
- 浓度：
0.4 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-PRCP antibody
Cat. No. JLC221156
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-PRCP rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human PRCP
Reactivity Human, Mouse
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PRCP
Full name Prolylcarboxypeptidase (angiotensinase C)
Synonyms PCP; HUMPCP
Swissprot P42785
Target Background
The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human ovarian cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221156(PRCP Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml221156(PRCP Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:56 kDa
Positive control:SKOV3, A549 cells and human liver cancer tissue
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 50 μg
Lane 1-3: SKOV3 cells, A549 cells, human liver cancer tissue
Primary antibody: ml221156(PRCP Antibody) at dilution 1/100
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
ELISA
Recommended dilution: 1000-2000
Cat. No. JLC221156
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-PRCP rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human PRCP
Reactivity Human, Mouse
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PRCP
Full name Prolylcarboxypeptidase (angiotensinase C)
Synonyms PCP; HUMPCP
Swissprot P42785
Target Background
The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human ovarian cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221156(PRCP Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml221156(PRCP Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:56 kDa
Positive control:SKOV3, A549 cells and human liver cancer tissue
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 50 μg
Lane 1-3: SKOV3 cells, A549 cells, human liver cancer tissue
Primary antibody: ml221156(PRCP Antibody) at dilution 1/100
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
ELISA
Recommended dilution: 1000-2000
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Anti-PRCP antibody
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