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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO
- 规格：
200ul
描述
The cell-impermeant viability indicator ethdium homodimer-2 (EthD-2) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~535/624).规格
| Color: | Orange |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 535⁄624 |
| Product Size: | 200 µL |
| Shipping Condition: | Room Temperature |
化学结构
分子式: C51H60I4N8
分子量: 1292.71
CAS名称/编号:
参考文献及引用
Int J Androl (2005) 28:107-114
J Biol Chem (2003) 278:18207-18213
Am J Respir Cell Mol Biol (2004) 30:605-612
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
in PBS. 2) Add 20 μl of 2 mM EthD-1 stock solution to 10 mL of sterile PBS and vortex to ensure thorough mixing. 3) Add 5 μl of 4 mM calcein AM stock stolution to EthD-1 solution. Again, vortex to ensure thorough mixing
(top rows). Live/dead discrimination was performed using calcein AM (green) to mark viable cells, and ethidium bromide homodimer‐1 (red) to identify dead cells. Scale bars represents 20 µm. Panel A was adapted from Cohen, et al. () with permission
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