- ￥3120
- biorbyt
- orb499583
- 英国
- 2025年12月03日
- IF, IHC-Fr, IHC-P, WB
- Mouse
- Human, Rat
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- 详细信息
- 技术资料
- 抗体名：
CK18 Mouse Monoclonal Antibody抗体
- 抗体英文名：
CK18 Mouse Monoclonal Antibody
- 靶点：
KRT18
- 浓度：
1mg/ml
- 应用范围：
IF, IHC-Fr, IHC-P, WB
- 宿主：
Mouse
- 适应物种：
Human, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb499583
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Recombinant human CK18
- 规格：
50 ul
产品描述：CK18 Mouse Monoclonal Antibody
别名:K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;
免疫原：Recombinant human CK18
预测反应性:Mouse
克隆性:3C3
分子量:48 kDa
应用稀释比例：WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P05783
Note:For research use only.
别名:K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;
免疫原：Recombinant human CK18
预测反应性:Mouse
克隆性:3C3
分子量:48 kDa
应用稀释比例：WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P05783
Note:For research use only.
paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (orb499583) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: A431 Cell (Human) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
Sample: Lane 1: Small intestine (Rat) Lysate at 40 ug, Lane 2: Pancreas (Rat) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Lane 4: Uterus (Rat) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 45 kD.
Sample: MCF-7 Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-CK18 (orb499583) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
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CK18 Mouse Monoclonal Antibody抗体，orb499583，biorbyt
￥3120
