- ￥3120
- biorbyt
- orb704519
- 英国
- 2025年12月05日
- ICC, WB
- Mouse
- Human
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- 详细信息
- 技术资料
- 抗体名：
CK18 Mouse Monoclonal Antibody抗体
- 抗体英文名：
CK18 Mouse Monoclonal Antibody
- 靶点：
KRT18
- 浓度：
1mg/ml
- 应用范围：
ICC, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb704519
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human CK18
- 规格：
50 ul
产品描述：CK18 Mouse Monoclonal Antibody
别名:K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;
免疫原：KLH conjugated synthetic peptide derived from human CK18
预测反应性:Mouse
克隆性:4H7
分子量:48 kDa
应用稀释比例：WB=1:500-1000, ICC/IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P05783
Note:For research use only.
别名:K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;
免疫原：KLH conjugated synthetic peptide derived from human CK18
预测反应性:Mouse
克隆性:4H7
分子量:48 kDa
应用稀释比例：WB=1:500-1000, ICC/IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P05783
Note:For research use only.
Sample: Lane 1: Hela (Human) cell Lysate at 30 ug, Lane 2: HepG2 (Human) cell Lysate at 30 ug, Lane 3: A549 (Human) cell Lysate at 30 ug, Lane 4: MCF-7 (Human) cell Lysate at 30 ug, Primary: Anti-CK18 (orb704519) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 45 kD.
Tissue/Cell: Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CK18) monoclonal Antibody, Unconjugated (orb704519) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CK18) monoclonal Antibody, Unconjugated (orb704519) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CK18) monoclonal Antibody, Unconjugated (orb704519) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
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CK18 Mouse Monoclonal Antibody抗体，orb704519，biorbyt
￥3120
