- $875
- LSBio
- 78 - 5000 pg/ml
- LS-F13176
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
78 - 5000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
Performance 100% Guaranteed
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Product Description
LS-F13176 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human F12 / Factor XII in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of F12 / Factor XII as low as 78 picograms per milliliter.More F12 / Factor XII Products
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
F12 / Factor XII
Synonyms
F12, Beta-factor XIIa part 2, Beta-factor XIIa part 1, Coagulation factor XII, F12 deficiency, Factor XII, Factor XII deficiency, HAE3, HAEX, Hageman factor, HAF
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78 - 5000 pg/ml
Sensitivity
78
Precision
Intra-Assay: CV<6.1% / Inter-Assay: CV<8.9%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About F12 / Factor XII
F12 / Factor XII is coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. P00748 NM_000505NP_000496.2
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Sandwich ELISA Platform Overview
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Rinse with PSA 3x 0.3% hydrogen peroxide in PBS, 30min. Then rinse in PBS 3x 5% normal goad serum in PBS at RT for 1 hour. Rabbit anti-Human Factor VIII Related Antigen diluted to 1:500 with 1% NGS in PBS, incubated
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