蓝色葡聚糖2000

蓝色葡聚糖2000是用于检测过滤柱装填是否合格。

蓝色葡聚糖 2000 Dextran Blue 2000

CAS Number: 87915-38-6

Storage Temperature: Room Temperature

Product Description :

The most common use for Blue Dextran is in the determination of the void volume in gel filtration chromatography columns. It is typically used at 1 mg/ml to 2 mg/ml in a buffer such as 50 mM Tris-HCl, pH 7.5 containing 100 mM KCl. The volume of the Blue Dextran sample should be 1% to no more than 3% of the column's bed volume. The shape of the Blue Dextran band as it elutes through the column can also be used to assess the column packing and resolution.

Blue Dextran has also been used as a spectrophotometric substrate for dextranase. Dextranase activity is measured by the increase in absorbance at 610 nm.

The affinity of the Reactive Blue 2 Dye in Blue dextran for certain proteins has been exploited in protein purification methods. When bound to a protein the Blue Dextran-protein complex will be eluted in the void volume of a sephadex gel filtration column. This allows the bound protein to be easily separated from other proteins of similar molecular weight. The binding of blue dextrans to proteins is dependent on pH and ionic strength.