- ￥8700
- Roche
- wlb1171
- China
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
In Situ Cell Death Detection Kit, POD
- CAS号：
wlb1171
- 保质期：
1年
- 供应商：
瓦兰生物
- 保存条件：
-20°
- 规格：
50T
产品简介
| 英文名称 | In Situ Cell Death Detection Kit, POD |
| 储存条件 | -20℃保存，有效期1年 |
| 规格 | 50T |
| 单位 | 盒 |
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Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method
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Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary detection system
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Convenient: The direct labeling procedure using fluorescein-dUTP allows verification of the eff *iciency of the TUNEL reaction during the assay procedure
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Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis
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Flexible: No substrate included; provides the opportunity to select the staining procedure of choice
Contents
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Enzyme Solution (TdT)
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Label Solution (fluorescein-dUTP)
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Converter POD (anti-fluorescein antibody-POD), ready-to-use
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文献和实验ziweihua 求助高人啊，我想用斑马鱼整体胚胎检测其细胞凋亡情况，选用的Roche的in situ cell death detection kit, POD. 按照其中的贴壁细胞或细胞涂片的方法做了两遍，可是连阳性对照都没有信号。后来查找到一篇论文中的方法，如下： a，收集胚胎后用PBS清洗，转移到1.5ml的EP管内，4%多聚甲醛PBS固定过 夜 b，用PBS配制 25%50%75%的梯度酒精，每次5min将胚胎梯度脱水。
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
information Moreover, as this procedure is quite complex, the use of commercial kits such as ApoTagTM (Oncor, Gaithersburg, MD, USA) and "In situ cell death detection Kit" (Boeringer-Mannheim, Germany), is recommended. Utilizing
Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transfer(TdT)
Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during apoptosis. Incorporation of biotinylated dUTP
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