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100ml
Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. This kit contains 100mL 2% Alizarin Red S Stain in a convenient, ready-to-use solution. The dye forms a complex with calcium during the process of chelation resulting in birefringence [1].
Materials Supplied by User
- 4% Paraformaldehyde solution in Phosphate Buffered Saline (PBS)
- Deionized H2O (diH2O)
| Catalog No. | 0223 |
|---|---|
| Country of Manufacture | United States |
| Product Code | ARed |
| Size/Quantity | 100 ml |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Room Temperature |
| Shipping Info | Ambient temperature. |
| References | [1 ] Clark, G. (Ed.).(1981). Staining Procedures. (4th ed.) Baltimore, MD: Williams & Wilkins. |
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文献和实验Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons
, kidneys and gut. - Alcian Blue (20mls) 2mgs alcian blue, dissolve in 0.8ml H20 add 16ml Absolute ETOH and 4mls glacial acetic acid. - Alizarin Red S Dissolve 1mg of alizarin red S in 100ml of 1% KOH. Disclaimer
Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons.
to alizarin red S/KOH solution, until bone is stained purple. 2-3 days. (This solution can be changed daily). Rinse in 1% KOH. ~ 3 changes several hours each. 20% Glycerol in 1% KOH 24hrs (swirl occasionally) 50% Glycerol
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
, and Ki-67 antibodies), while the staining of many other antibodies may be enhanced by epitope retrieval protocol (e.g, S-100, cytokeratin, and synaptophysin antibodies). You may use any other epitope retrieval protocol recommended by the supplier
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