- $715
- LSBio
- 0.312 - 20 ng/ml
- LS-F10113
- 美国
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.312 - 20 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human EGFR ELISA Kit (Sandwich ELISA) - LS-F10113
Performance 100% Guaranteed
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Rat EGFR ELISA Kit (Sandwich ELISA) - LS-F5030
Reactivity: Rat
Range: 0.313-20 ng/ml
Human EGFR ELISA Kit (Sandwich ELISA) - LS-F13162
Reactivity: Human
Range: 78-5000 pg/ml
Human Phospho-EGFR ELISA Kit - LS-F334
Reactivity: Human
Human EGFR ELISA Kit (Sandwich ELISA) - LS-F70
Reactivity: Human
Range: 2.743-666.7 pg/ml
More EGFR Products
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Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
EGFR
Synonyms
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human EGFR. No significant cross-reactivity or interference between human EGFR and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human EGFR and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.019 ng/ml
Precision
Intra-assay: CV%<8% Inter-assay: CV%<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2
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Sandwich ELISA Platform Overview
Performance 100% Guaranteed
Print This Page
Technical Help
Review This Product
SpecificationsPublicationsReviewsImages
Popular EGFR Elisa Kits
Rat EGFR ELISA Kit (Sandwich ELISA) - LS-F5030
Reactivity: Rat
Range: 0.313-20 ng/ml
Human EGFR ELISA Kit (Sandwich ELISA) - LS-F13162
Reactivity: Human
Range: 78-5000 pg/ml
Human Phospho-EGFR ELISA Kit - LS-F334
Reactivity: Human
Human EGFR ELISA Kit (Sandwich ELISA) - LS-F70
Reactivity: Human
Range: 2.743-666.7 pg/ml
Product Description
LS-F10113 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human EGFR in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EGFR as low as 0.019 nanograms per millilter.More EGFR Products
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
EGFR
Synonyms
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human EGFR. No significant cross-reactivity or interference between human EGFR and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human EGFR and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.019 ng/ml
Precision
Intra-assay: CV%<8% Inter-assay: CV%<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2
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Submit a Review
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Images
Sandwich ELISA Platform Overview
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Human EGFR ELISA Kit (Sandwich ELISA) - LS-F10113
$715
