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- BIO-RAD
- 1652082
- 美国
- 2025年07月15日
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1652082
Use the 0.2 cm–gap Gene Pulser/MicroPulser Electroporation Cuvettes for reproducible transformation of bacteria, yeast, and mammalian cells, including primary and stem cells. These high-quality cuvettes are compatible with most electroporation systems, including Bio-Rad’s Gene Pulser MXcell™, Gene Pulser Xcell™, and MicroPulser Electroporators.
Gene Pulser/MicroPulser Cuvettes are available in three gap widths:
- 0.1 cm (brown cap) – for bacteria and yeast
- 0.2 cm (green cap) – for bacteria, yeast, and mammalian cells, including primary and stem cells
- 0.4 cm (blue cap) – for mammalian cells, including primary and stem cells, or plant protoplasts
Packaging Options
| Gap Size | Package Size | Catalog Number |
| 0.2 cm | 5 | 1652082 |
| 0.2 cm | 50 | 1652086 |
| 0.2 cm | 500 | 1652092 |
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文献和实验Transformation of E. coli by Electroporation
electroporation cuvette (0.2-cm gap) and test whether arcing occurs when an electrical discharge is applied (please see Step 16 below). If so, wash the remainder of the cell suspension once more with ice-cold GYT medium to ensure that the conductivity
Stable Transfection (Electroporation)
medium. Supplies & Equipment: BioRad gene pulser with capacitance extender Electroporation cuvettes with 0.4 cm interelectrode distance Reagents: sterile 1 x HeBS buffer (Hepes buffered saline) G 418 (Geneticin
% confluent) should be passaged 1:2 the day before electroporation. Cells to be electroporated should be fed 4 hours before harvesting. 2. Trypsinize cells and resuspend in media (cells from 2 x 10 cm plates can be combined in a total volume of 10
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