- 询价
- BIO-RAD
- 1708891
- 美国
- 2026年01月13日
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- 现货状态：
现货
- 供应商：
BIO-RAD
- 规格：
1708891
Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
- Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Fast 26 min cDNA synthesis protocol
- Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, µl | Catalog Number |
| 25 | 100 (1 x 100 µl) | 1708890 |
| 100 | 400 (1 x 400 µl) | 1708891 |
| 500 | 2,000 (5 x 400 µl) | 1708891BUN |
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文献和实验of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
Reverse Transcription of RNA- 20l rxn
0.01l is the 1st strand derived from 0.00075 ug of Poly A+ RNA which is the equivalent of 0.15ug of total RNA (X-H''s method works out to use the eqivalent of 0.12ug of total RNA in the RT-PCR) Controls should include: 1) primers alone
cDNA synthesis using superscript II
1 µg random hexamers (100 ng/µl) 25 ng/µl 5 µl water add to 20 µl Anneal the primers in a thermocycler
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