- 询价
- BIO-RAD
- 1725125
- 美国
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
BIO-RAD
- 保存条件：
低温
- 规格：
1725125
Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).
- Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument
- Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)
- Amplify genomic DNA and difficult amplicons
- Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Instrument Compatibility
iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
| Systems | |
| Applied Biosystems |
|
| Stratagene |
|
| Eppendorf |
|
| QIAGEN |
|
| Roche |
|
| BioFire |
|
* BSA must be added according to instrument specifications
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 1725120 |
| 500 | 5 x 1 | 1725121 |
| 1,000 | 10 x 1 | 1725122 |
| 2,500 | 5 x 5 | 1725124 |
| 5,000 | 10 x 5 | 1725125 |
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文献和实验Clonality - X Chromosome Inactivation Assay
microdissected cells in 20 µl proteinase K buffer. Incubate overnight at 37°C. B: DNA Purification Lyse the cells overnight at 42°C, in 50 µl phenol:chloroform:isoamyl alcohol. Boil 10 min at 95°C. Vortex 1 min. Centrifuge 20 min at 14,000 rpm at RT
Vybrant® DyeCycle Green and Orange Stains
add 2 μL of Vybrant® DyeCycle™ Green stain or Vybrant® DyeCycle™ Orange stain. Final stain concentration is 10 μM. After use, seal the stain vial tightly. The stain in DMSO solution may be subjected to many freeze-thaw cycles without reagent
SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
