丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片

丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片英文名称  Anti-PIM1+PIM3
中文名称  丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片
别    名 OncogenePIM1;OncogenePIM1;PIM1;pim1kinase44kDaisoform;Pim1kinase;pim1oncogene(proviralintegrationsite1);Pim1oncogene;PIM;PIM1;pim1kinase44kDaisoform;PIM1_HUMAN;Pim2;PIM3;Protooncogeneserine/threonineproteinkinasePim1;Proto-oncogeneserine/threonine-proteinkinasePim-1;Proviralintegrationsite1;Proviralintegrationsite2;Kid1;Kid-1;Kid1;Kinaseinducedbydepolarization;ONCOGENEPIM3;ONCOGENEPIM3;PIM3;Pim3oncogene;PIM3;Pim3oncogene;PIM3_HUMAN;ProteinkinaseKid1;ProteinkinaseKid1;Serine/threoninekinasePim3;Serine/threoninekinasePim3;Serine/threonineproteinkinasePim3;Serine/threonineproteinkinasePim3;Serine/threonine-proteinkinasePim-3.

浓度1mg/1ml
规格0.2ml/200μg
抗体来源Rabbit
克隆类型polyclonal
交叉反应Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig
产品类型一抗
研究领域
蛋白分子量predictedmolecularweight：
45kDa 
性状LyophilizedorLiquid
免疫原KLHconjugatedsyntheticpeptidederivedfromhumanPIM1+PIM3
亚型IgG
纯化方法affinitypurifiedbyProteinA
储存液Preservative:15mMSodiumAzide,Constituents:1%BSA,0.01MPBS,pH7.4
产品应用WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500
（石蜡切片需做抗原修复）
notyettestedinotherapplications.
optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
保存条件Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
ImportantNoteThisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
产品介绍PIM1isamemberoftheserine/threoninekinasePIMoncogenefamily.PIM1hasbeenimplicatedinlymphomagenesis,cellproliferation,apoptosis,differentiationandtumourigenesis.ThePIM1proteinkinaseisupregulatedinprostatecancer.
ThePimfamilyserine/threonineproteinkinaseswerefirstidentifiedinstudiesexamininggenestargetedforproviralinsertioninmurineleukemiavirus-inducedTlymphomas.IncreasedlevelsofPimkinasespredisposecellstolymphomagenesisandenhancetheactivityofmitogenicproteinssuchasp100,c-Myb,andCdc25A.Inaddition,Pimkinasesarealsoinvolvedinmodulationofsynapticstrengthinneuronsandanti-apoptoticsignalinginhematopoieticprogenitorcells.Pim-3,amemberoftheproto-oncogenePimfamilythatexpressesserine/threoninekinaseactivity,sharessignificanthomologywithPim-1serine/threonineproteinkinases.Pim-3mayfunctionasamediatorofsynapticplasticityinthebrainandispresumablyinvolvedintheanti-apoptosisprocessandcellcycleprogressionaswellastheproliferationofhumanhepatomacelllines.ThePim-3proteiniswidelyexpressed,howevernoexpressionisobservedinthecolon,thymus,orsmallintestine.
Function:Proto-oncogenewithserine/threoninekinaseactivityinvolvedincellsurvivalandcellproliferationandthusprovidingaselectiveadvantageintumorigenesis.Exertsitsoncogenicactivitythrough:theregulationofMYCtranscriptionalactivity,theregulationofcellcycleprogressionandbyphosphorylationandinhibitionofproapoptoticproteins(BAD,MAP3K5,FOXO3).PhosphorylationofMYCleadstoanincreaseofMYCproteinstabilityandtherebyanincreaseoftranscriptionalactivity.ThestabilizationofMYCexercedbyPIM1mightexplainpartlythestrongsynergismbetweenthesetwooncogenesintumorigenesis.MediatessurvivalsignalingthroughphosphorylationofBAD,whichinducesreleaseoftheanti-apoptoticproteinBcl-X(L)/BCL2L1.PhosphorylationofMAP3K5,anotherproapoptoticprotein,byPIM1,significantlydecreasesMAP3K5kinaseactivityandinhibitsMAP3K5-mediatedphosphorylationofJNKandJNK/p38MAPKsubsequentlyreducingcaspase-3activationandcellapoptosis.StimulatescellcycleprogressionattheG1-SandG2-MtransitionsbyphosphorylationofCDC25AandCDC25C.PhosphorylationofCDKN1A,aregulatorofcellcycleprogressionatG1,resultsintherelocationofCDKN1AtothecytoplasmandenhancedCDKN1Aproteinstability.Promotecellcycleprogressionandtumorigenesisbydown-regulatingexpressionofaregulatorofcellcycleprogression,CDKN1B,atbothtranscriptionalandpost-translationallevels.PhosphorylationofCDKN1B,induces14-3-3-proteinsbinding,nuclearexportandproteasome-dependentdegradation.MayaffectthestructureorsilencingofchromatinbyphosphorylatingHP1gamma/CBX3.ActsalsoasaregulatorofhomingandmigrationofbonemarrowcellsinvolvingfunctionalinteractionwiththeCXCL12-CXCR4signalingaxis.
Subunit：
Isoform2isisolatedasamonomerwhereasisoform1complexeswithotherproteins.BindstoRP9.Isoform1,butnotisoform2,bindsBMX.Isoform2interactswithCDKN1BandFOXO3.InteractswithBAD.InteractswithPPP2CA;thisinteractionpromotesdephosphorylationofPIM1,ubiquitinationandproteasomaldegradation.InteractswithHSP90,thisinteractionstabilizesPIM1proteinlevels.Interacts(ubiquitinatedform)withHSP70andpromotesitsproteosomaldegradation.InteractswithCDKN1A.InteractswithCDC25C.Interacts(viaN-terminal96residues)withCDC25A.InteractswithMAP3K5.InteractswithMYC. 
SubcellularLocation:Isoform2:Cytoplasm.Nucleus.Isoform1:Cellmembrane.
TissueSpecificity：
Expressedprimarilyincellsofthehematopoieticandgermlinelineages.Isoform1andisoform2arebothexpressedinprostatecancercelllines. 
Post-translationalmodifications：
Autophosphorylatedonbothserine/threonineandtyrosineresidues.Phosphorylated.InteractionwithPPP2CApromotesdephosphorylation. 
Ubiquitinated,leadingtoproteasomaldegradation.
Similarity：
Belongstotheproteinkinasesuperfamily.CAMKSer/Thrproteinkinasefamily.PIMsubfamily. 
Contains1proteinkinasedomain.
Databaselinks:UniProtKB/Swiss-Prot:Q86V86.3UniProtKB/Swiss-Prot:P11309.3

丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片抗体的生物素化标记实验要点：
1.如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L缓冲液充分透析；
2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；
3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；
4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；
5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；
6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及基,也可与糖基共价结合；
7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；
8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。
丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
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Immunoprecipitation/IPKit-Anti-Peroxiredoxin1/PRDX1ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-CSRP1ImmunomagneticBeadsIP   5mL
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Immunoprecipitation/IPKit-Anti-S100A7ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-S100A10ImmunomagneticBeadsIP   5mL
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Immunoprecipitation/IPKit-Anti-AnnexinVI/ANXA6ImmunomagneticBeadsIP   5mL
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Immunoprecipitation/IPKit-Anti-LRPAP1ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-SerpinF1/PEDFImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-ACBD6ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-S100A16ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-S100A11/S100CImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-LRPAP1ImmunomagneticBeadsIP   5mL
Immunoprecipitation/IPKit-Anti-LRPAP1ImmunomagneticBeadsIP   5mL
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Immunoprecipitation/IPKit-Anti-HAImmunomagneticBeadsIP   5mL
丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片菌种保存和标本运送培养基
StrainStoreMedium瓷珠菌种保存管（50支）50支/盒
StrainStoreMedium瓷珠菌种保存管（20支）20支/盒
AmiesTransportMediumAmies运送培养基管20支/包
StuartTransportMediumStuart运送培养基管20支/包
Cary-BlairTransportMediumCary-Blair氏运送培养基管20支/包
MEMmaintenanceoffluidMEM维持液36支/盒
无菌生理盐水管（内附采样棉签）
丝氨酸/苏氨酸激酶蛋白PIM1+PIM3抗体图片技术外包服务:
★分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等
★蛋白工程：原核、哺乳动物蛋白表达系统等
★病毒包装：腺病毒、慢病毒等
★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。