NaHCO3(sodium bicarbonate) 37.

TransWell Chemotaxis

in 37°C equilibrated C-Buffer at a concentration of 1 X 106 cells / 100 μl (see Hint #5 ). 7. Return the Transwell® filter inserts from Step #2 to the wells containing the chemoattractant dilutions. Add 100 μl of the cell suspension

p27 ELISA

Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells

盐溶液配制

/w) 9.646 17.797 H2 O %(w/w) 90.354 82.203 p (g/ml) 1.0989 1.1912 NaHCO3 Sodium Bicarbonate, NaHCO3 , Mw=84.01g/M; (store at NT). Conc. Stock %(w/w) 50ml 100ml 250ml NaHCO3 1M