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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-4509R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-CXCL13/BCA1/FITC
- 抗体名:
Anti-CXCL13/BCA1/FITC
- 标记物:
FITC标记
- 宿主:
Mouse, Rat,
- 适应物种:
Mouse, Rat,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-CXCL13/BCA1/FITC |
| 中文名称 | FITC标记的B-淋巴细胞趋化因子抗体 |
| 别 名 | C-X-C motif chemokine 13; Angie; ANGIE; ANGIE2; B cell-attracting chemokine 1; B cell attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); CXL13_MOUSE; BCA-1; BLC; BLR1L; Chemokine (C-X-C motif) ligand 13; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; SCYB13; Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small-inducible cytokine B13; Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 趋化因子 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 10kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CXCL13/BCA1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. Function: Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs. Subcellular Location: Secreted. Tissue Specificity: Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles). Similarity: Belongs to the intercrine alpha (chemokine CxC) family. Database links: Entrez Gene: 10563 Human Entrez Gene: 55985 Mouse Entrez Gene: 498335 Rat Omim: 605149 Human SwissProt: O43927 Human SwissProt: O55038 Mouse Unigene: 100431 Human Unigene: 10116 Mouse Unigene: 6917 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验14圈定单核细胞2、荧光标记的细胞因子抗体R&D提供FITC、PE和APC标记的细胞因子抗体3、溶血素用外周全血检测时需使用溶血素(R&D目录号WL1000用于人或者WL2000用于小鼠;eBioscience目录号00-4333)溶解红细胞4、激活剂① Phorbol 12-Myristate 13 Acetate(PMA)(Alexis,目录号ALX-445-004-M001)DMSO中调节浓度0.1mg/mL分装(20L),-20℃储存。勿反复冻融每次实验用无菌无叠氮钠PBS 1:100稀释
择同型对照? (1)一般选择与一抗的成分完全相同种属来源、相同亚型和亚链、相同荧光标记的抗体,比如抗人的CD56 FITC标记的抗体,成份是MouseIgG1,κ。那么它的同型对照应该是FITC标记的MouseIgG1,κ。注意同型对照的成份跟它的名称是相同的. (2)如果是抗体的组合形式是纯化的一抗+荧光标记的二抗,那么应该选择一抗的同型对照。比如CD86的纯化抗体,成分是MouseIgG1,κ。它的同型对照是纯化的Mouse IgG1,κ。它的二抗PE标记的抗
CD56圈定NK淋巴细胞 CD14圈定单核细胞 2、荧光标记的细胞因子抗体 R&D提供FITC、PE和APC标记的细胞因子抗体 3、溶血素 用外周全血检测时需使用溶血素(R&D目录号WL1000用于人或者WL2000用于小鼠;eBioscience目录号00-4333)溶解红细胞 4、激活剂 ① Phorbol 12-Myristate 13 Acetate(PMA)(Alexis,目录号ALX-445
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