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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
详见官方网站
- 亚型:
详见官方网站
- 形态:
冻干粉/浓缩液
- 保存条件:
-20℃
- 克隆性:
多克隆
- 标记物:
详见说明书
- 适应物种:
详见说明书
- 宿主:
详见官方网站
- 应用范围:
详见官方网站
- 浓度:
1mg/ml
- 抗体英文名:
Ghrelin, 24-117 aa, Recombinant Protein
- 规格:
100ug:286,500ug:858
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文献和实验Detection of Protein‐Protein Interactions by Coprecipitation
of interest is precipitated, and the precipitate is tested for a second specifically associated protein. The approach can be used for native or epitope?tagged proteins for which antibodies are available, or for recombinant proteins that bind with high affinity to a molecule
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
Tris 300mM NaCl 0.87 g NaCl 8.0M Urea 24 g Urea 100mM Imidazole 0.33 g Imidazole H2O to 50 ml finalRenaturation of BRF protein Thaw Ni-NTA purified samples.Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer
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