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- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 详见说明书
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
磷酸化丝裂原活化蛋白激酶激酶1抗体
- 抗体英文名：
Anti-phospho-MEK1(Thr286)
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海谷研
- 库存：
57
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr286
- 规格：
0.1ml/100μg
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg现货供应，货期短，为您提供售前、售中、售后服务，保证实验结果，无效可以免费退换，提供免费代测服务，产品效价高、种类全、价格实惠 ，欢迎来电咨询！
英文名称 Anti-phospho-MEK1(Thr286)
中文名称 磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg
别 名 Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight：43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr286
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Function ：
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Subunit ：
Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce.
Subcellular Location ：
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg抗体的多样性：
抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg实验步骤
① 滴加0.01mol/L，pH7.4的PBS于待检标本片上，10min后弃去，使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液，使其完全覆盖标本，置于有盖搪瓷盒内，保温一定时间（参考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS冲洗后，再按顺序过0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不时振荡。
④ 取出玻片，用滤纸吸去多余水分，但不使标本干燥，加一滴缓冲甘油，以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度，一般可用“+”表示：
（-）无荧光；（±）极弱的可疑荧光；（+）荧光较弱，但清楚可见；（++）荧光明亮；（+++ --++++）荧光闪亮。待检标本特异性荧光染色强度达“++”以上，而各种对照显示为（±）或（-），即可判定为阳性。
注意事项
1）对荧光标记的抗体的稀释，要保证抗体的蛋白有一定的浓度，一般稀释度不应超过1：20，抗体浓度过低，会导致产生的荧光过弱，影响结果的观察。
2）染色的温度和时间需要根据各种不同的标本及抗原而变化，染色时间可以从10 min到数小时，一般30 min已足够。染色温度多采用室温（25℃左右），高于37℃可加强染色效果，但对不耐热的抗原（如流行性乙型脑炎病毒）可采用0-2℃的低温，延长染色时间。低温染色较37℃30 min效果好的多。
3）为了保证荧光染色的正确性，首次试验时需设置下述对照，以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照：标本加1-2滴0.01mol/L，pH7.4的PBS。
② 特异性对照（抑制试验）：标本加未标记的特异性抗体，再加荧光标记的特异性抗体。
③ 阳性对照：已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光，阳性对照和待检标本呈强荧光，则为特异性阳性染色。
4）一般标本在高压汞灯下照射超过3min，就有荧光减弱现象，经荧光染色的标本zui好在当天观察，随着时间的延长，荧光强度会
特价促销 TKN1 Polyclonal Antibody 速激肽1 多克隆抗体
特价促销 THNS2 Polyclonal Antibody 苏氨酸合成酶样蛋白2 多克隆抗体
特价促销 REL2 Polyclonal Antibody 松弛肽/松弛素2 多克隆抗体
特价促销 REL1 Polyclonal Antibody 松弛肽/松弛素1 多克隆抗体
特价促销 RXFP1 Polyclonal Antibody 松弛素/胰岛素样肽受体1 多克隆抗体
特价促销 TPRX1 Polyclonal Antibody 四肽重复同源框1 多克隆抗体
特价促销 DEDD Polyclonal Antibody 死亡效应结构域蛋白 多克隆抗体
特价促销 CRADD Polyclonal Antibody 死亡结构域含蛋白CRADD 多克隆抗体
特价促销 STC2 Polyclonal Antibody 斯钙素蛋白2 多克隆抗体
特价促销 STC1 Polyclonal Antibody 斯钙素蛋白1 多克隆抗体
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg5(6)-羧基荧光素二乙酸盐CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers*
5(6)-羧基二乙酸荧光素琥珀酰亚胺酯CFDA, SE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *Mixed isomers*
5(6)-羧基-2',7'-二氯荧光素二乙酸盐CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*
5(6)-羧基-2',7'-二氯二乙酸荧光素琥珀酰亚胺酯CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*
JC-1JC-1 [5,5‘,6,6’-Tetrachloro-1,1‘,3,3’-tetraethylbenzimidazolylcarbocyanine iodide]
JC-1JC-1 [5,5‘,6,6’-Tetrachloro-1,1‘,3,3’-tetraethylbenzimidazolylcarbocyanine iodide]
线粒体膜电位检测试剂盒(JC-1)Mitochondrial Membrane Potential Assay Kit with JC-1
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好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
英文名称 Anti-phospho-MEK1(Thr286)
中文名称 磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg
别 名 Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight：43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr286
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Function ：
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Subunit ：
Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce.
Subcellular Location ：
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg抗体的多样性：
抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg实验步骤
① 滴加0.01mol/L，pH7.4的PBS于待检标本片上，10min后弃去，使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液，使其完全覆盖标本，置于有盖搪瓷盒内，保温一定时间（参考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS冲洗后，再按顺序过0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不时振荡。
④ 取出玻片，用滤纸吸去多余水分，但不使标本干燥，加一滴缓冲甘油，以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度，一般可用“+”表示：
（-）无荧光；（±）极弱的可疑荧光；（+）荧光较弱，但清楚可见；（++）荧光明亮；（+++ --++++）荧光闪亮。待检标本特异性荧光染色强度达“++”以上，而各种对照显示为（±）或（-），即可判定为阳性。
注意事项
1）对荧光标记的抗体的稀释，要保证抗体的蛋白有一定的浓度，一般稀释度不应超过1：20，抗体浓度过低，会导致产生的荧光过弱，影响结果的观察。
2）染色的温度和时间需要根据各种不同的标本及抗原而变化，染色时间可以从10 min到数小时，一般30 min已足够。染色温度多采用室温（25℃左右），高于37℃可加强染色效果，但对不耐热的抗原（如流行性乙型脑炎病毒）可采用0-2℃的低温，延长染色时间。低温染色较37℃30 min效果好的多。
3）为了保证荧光染色的正确性，首次试验时需设置下述对照，以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照：标本加1-2滴0.01mol/L，pH7.4的PBS。
② 特异性对照（抑制试验）：标本加未标记的特异性抗体，再加荧光标记的特异性抗体。
③ 阳性对照：已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光，阳性对照和待检标本呈强荧光，则为特异性阳性染色。
4）一般标本在高压汞灯下照射超过3min，就有荧光减弱现象，经荧光染色的标本zui好在当天观察，随着时间的延长，荧光强度会
特价促销 TKN1 Polyclonal Antibody 速激肽1 多克隆抗体
特价促销 THNS2 Polyclonal Antibody 苏氨酸合成酶样蛋白2 多克隆抗体
特价促销 REL2 Polyclonal Antibody 松弛肽/松弛素2 多克隆抗体
特价促销 REL1 Polyclonal Antibody 松弛肽/松弛素1 多克隆抗体
特价促销 RXFP1 Polyclonal Antibody 松弛素/胰岛素样肽受体1 多克隆抗体
特价促销 TPRX1 Polyclonal Antibody 四肽重复同源框1 多克隆抗体
特价促销 DEDD Polyclonal Antibody 死亡效应结构域蛋白 多克隆抗体
特价促销 CRADD Polyclonal Antibody 死亡结构域含蛋白CRADD 多克隆抗体
特价促销 STC2 Polyclonal Antibody 斯钙素蛋白2 多克隆抗体
特价促销 STC1 Polyclonal Antibody 斯钙素蛋白1 多克隆抗体
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg5(6)-羧基荧光素二乙酸盐CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers*
5(6)-羧基二乙酸荧光素琥珀酰亚胺酯CFDA, SE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *Mixed isomers*
5(6)-羧基-2',7'-二氯荧光素二乙酸盐CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*
5(6)-羧基-2',7'-二氯二乙酸荧光素琥珀酰亚胺酯CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*
JC-1JC-1 [5,5‘,6,6’-Tetrachloro-1,1‘,3,3’-tetraethylbenzimidazolylcarbocyanine iodide]
JC-1JC-1 [5,5‘,6,6’-Tetrachloro-1,1‘,3,3’-tetraethylbenzimidazolylcarbocyanine iodide]
线粒体膜电位检测试剂盒(JC-1)Mitochondrial Membrane Potential Assay Kit with JC-1
磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
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文献和实验相关实验
质芯片检测。此外，肽芯片或基于质谱的方法[ 20，21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白（MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片（见第 28 章）。在放射性【 γ33 磷】三磷酸腺苷存在的条件下，用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号，对可能
.8ml，巯基乙醇3.2ml，0.05%溴酚蓝1.6ml，H2O 32ml混匀备用。按1:1或1:2比例与蛋白质样品混合，在沸水终煮3min混匀后再上样，一般为20-25ul，总蛋白量100μg。7、 Tris-甘氨酸电泳缓冲液:30.3gTris，188g甘氨酸，10gSDS，用蒸馏水溶解至1000ml，得0.25mol/L Tris-1.92mol/L甘氨酸电极缓冲液。临用前稀释10倍。8、 转移缓冲液：配制1L转移缓冲液，需称取2.9g甘氨酸、5.8gTris碱、0.37g SDS，并加入200
%溴酚蓝1.6ml，H2O 32ml混匀备用。按1:1或1:2比例与蛋白质样品混合，在沸水终煮3min混匀后再上样，一般为20-25ul，总蛋白量100μg。7、 Tris-甘氨酸电泳缓冲液:30.3gTris，188g甘氨酸，10gSDS，用蒸馏水溶解至1000ml，得0.25mol/L Tris-1.92mol/L甘氨酸电极缓冲液。临用前稀释10倍。8、 转移缓冲液：配制1L转移缓冲液，需称取2.9g甘氨酸、5.8gTris碱、0.37g SDS，并加入200ml甲醇，加水至总量1L。9、 丽春
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磷酸化丝裂原活化蛋白激酶激酶1抗体0.1ml/100μg
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