- ￥1147
- worthington
- 美国
- LS004454
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at 2-8°C.
- 保质期：
详见说明书
- 英文名：
Trypsin, Sterile, Irradiated
- 库存：
大量现货供应
- 供应商：
上海经科化学科技有限公司
- CAS号：
TRLVMF
- 规格：
100 mg
Trypsin, Sterile, Irradiated
Chromatographically purified (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR113.53c. Each vial is filled to contain ≥100 mg.
名称：Trypsin, Sterile, Irradiated
货号：LS004454
规格：100 mg
价格：1147
Code：TRLVMF
Activity/Specification：≥180 units per mg protein (at least 10,350 BAEE/3450 USP/NF units per mg protein)
UNSPSC Code(s)*：12352204
Source：Bovine Pancreas
Storage Conditions：Store at 2-8°C..
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文献和实验, Trypsin solution GIBCO, Cat. gibco Water, sterile制备 Trypsin working solution Trypsin solution, 2.5% 1.5 ml Earles BSS 50 ml earles Fetal bovine serum solution Fetal bovine serum 1 ml Earles BSS 50ml earles
FEEDER PREPARATION BY GAMMA IRRADIATION
. 6. Add 5 - 6 ml of feeder media (DMEM, 7% FBS, 1XGPS) to each plate. This inactivates the trypsin. 7. Harvest cells by pipeting up and down the cell suspension and transfer to sterile 50 ml centrifuge tube. Repeat the process and pool
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, at embryonic day 13-15 (for Protocol 3; see Step 52) Mouse embryonic fibroblast (mEF) cells, irradiated and growing in a 6-cm dish (for Protocol 2) Alternatively, a gelatin-coated 6-cm dish can be used. For a protocol to isolate and maintain mEFs
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