- 询价
- worthington
- 美国
- LS04073
- 2025年07月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C.
- 保质期：
详见说明书
- 英文名：
Nuclease, S1
- 库存：
大量现货供应
- 供应商：
上海经科化学科技有限公司
- CAS号：
SINUC
- 规格：
Bulk
Nuclease, S1
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30 mM sodium acetate, pH 4.6, 50 mM NaCl, 1 mM ZnCl2, and 50% glycerol.
名称：Nuclease, S1
货号：LS04073
规格：Bulk
价格：询价
Code：SINUC
Activity/Specification：≥100,000 to 500,000 units per ml
UNSPSC Code(s)*：12352204
Source：Aspergillus oryzae
Storage Conditions：Store at -20°C..
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文献和实验S1 Nuclease Protection Mapping
The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp (1 ), it has undergone many refinements over the years. S1 nuclease mapping requires a relatively
4. Vortex vigorously. 5. Incubate at 100 deg C for 3 minutes. 6. Transfer immediately to 58 deg C and incubate overnight. S1 digestion buffer (final concentration): 300 mM NaCl 30 mM sodium acetate, pH 5.5 2 mM zinc sulfate 1000 units/ml S1 nuclease
S1 Nuclease Analysis of Alternatively Spliced mRNA
-PCR and nuclease protection assays are the most frequently performed methods in the laboratory. Here, we describe the analysis of alternatively spliced RNA by using 5′ -end labelled DNA oligonucleotide probes and S1 nuclease. The method is sensitive, allowing
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