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文献和实验S1 Nuclease Protection Mapping
The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp (1 ), it has undergone many refinements over the years. S1 nuclease mapping requires a relatively
4. Vortex vigorously. 5. Incubate at 100 deg C for 3 minutes. 6. Transfer immediately to 58 deg C and incubate overnight. S1 digestion buffer (final concentration): 300 mM NaCl 30 mM sodium acetate, pH 5.5 2 mM zinc sulfate 1000 units/ml S1 nuclease
S1 Nuclease Analysis of Alternatively Spliced mRNA
-PCR and nuclease protection assays are the most frequently performed methods in the laboratory. Here, we describe the analysis of alternatively spliced RNA by using 5′ -end labelled DNA oligonucleotide probes and S1 nuclease. The method is sensitive, allowing
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