- ¥1800
- PTGCN
- 14395-1-AP
- 中国
- 2025年12月20日
- 免疫组化
- 兔
- human, mouse, rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体名：
SMA免疫组化抗体
- 应用范围：
免疫组化
- 适应物种：
human, mouse, rat
- 保质期：
一年
- 抗原来源：
其它
- 供应商：
上海研卉生物科技有限公司
- 宿主：
兔
- 库存：
大量
- 是否单克隆：
多克隆
- 抗体英文名：
smooth muscle actin Antibody
- 规格：
100ul
SMA免疫组化抗体
smooth muscle actin Antibody
smooth muscle actin Antibody
Tested Applications
Applications:
WB, IP, IHC, IF, FC, ELISA
Species specificity:
human, mouse, rat
Positive WB detected in:
mouse colon tissue, mouse kidney tissue, human placenta tissue, mouse liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
C2C12 cell, mouse heart tissue, C2C12 cells
Positive FC detected in:
C2C12 cell
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:1500-1:6000
IF : 1:20-1:200
Synonyms
a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, α SMA
| Author: | Yu Ling L | |
| Pubmed ID: | 22848271 | |
| Journal: |
Oncol Lett
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| Title: |
Fusion between cancer cells and myofibroblasts is involved in osteosarcoma.
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|
| Application: | IHC | |
| Species: | human | |
| Author: | Bian Er-Bao EB | |
| Pubmed ID: | 24211420 | |
| Journal: |
Toxicol Lett
|
|
| Title: |
Repression of Smad7 mediated by DNMT1 determines hepatic stellate cell activation and liver fibrosis in rats.
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|
| Application: | WB | |
| Species: | rat | |
| Author: | Sun Xu X | |
| Pubmed ID: | 24242045 | |
| Journal: |
Mol Cell Biochem
|
|
| Title: |
Participation of miR-200a in TGF-β1-mediated hepatic stellate cell activation.
|
|
| Application: | WB,IHC | |
| Species: | rat | |
| Author: | Yun Zhou | |
| Pubmed ID: | 24578384 | |
| Journal: |
Arterioscler Thromb Vasc Biol
|
|
| Title: |
Overexpression of c1q/tumor necrosis factor-related protein-3 promotes phosphate-induced vascular smooth muscle cell calcification both in vivo and in vitro.
|
|
| Application: | WB | |
| Species: | rat | |
| Author: | Lei Wang | |
| Pubmed ID: | 24717226 | |
| Journal: |
Transplantation
|
|
| Title: |
Hyperthermia-Conditioned OECs Serum-Free-Conditioned Medium Induce NSC Differentiation Into Neuron More Efficiently by the Upregulation of HIF-1 Alpha and Binding Activity.
|
|
| Application: | ||
| Species: | ||
| Author: | Li Xu | |
| Pubmed ID: | 24784499 | |
| Journal: |
Exp Lung Res
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|
| Title: |
Effects and mechanism of dehydroepiandrosterone on epithelial-mesenchymal transition in bronchial epithelial cells.
|
|
| Application: | ||
| Species: | ||
| Author: | Ziming Yuan | |
| Pubmed ID: | 24795507 | |
| Journal: |
Mediators Inflamm
|
|
| Title: |
Regulating inflammation using Acid-responsive electrospun fibrous scaffolds for skin scarless healing.
|
|
| Application: | WB | |
| Species: | rat | |
| Author: | Hui Cao | |
| Pubmed ID: | 24953987 | |
| Journal: |
J Surg Res
|
|
| Title: |
Upregulation of let-7a inhibits vascular smooth muscle cell proliferation in vitro and in vein graft intimal hyperplasia in rats.
|
|
| Application: | IHC | |
| Species: | rat | |
| Author: | Yan Huang | |
| Pubmed ID: | 24961950 | |
| Journal: |
Mol Cell Biochem
|
|
| Title: |
Endoplasmic reticulum stress-induced hepatic stellate cell apoptosis through calcium-mediated JNK/P38 MAPK and Calpain/Caspase-12 pathways.
|
|
| Application: | WB | |
| Species: | rat | |
| Author: | Sun-Xia Chen | |
| Pubmed ID: | 25118038 | |
| Journal: |
J Proteomics
|
|
| Title: |
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.
|
|
| Application: | WB, IF | |
| Species: | human | |
| Author: | Yong-Heng Luo | |
| Pubmed ID: | 25144187 | |
| Journal: |
PLoS One
|
|
| Title: |
Rosiglitazone Inhibits TGF-β 1 Induced Activation of Human Tenon Fibroblasts via p38 Signal Pathway.
|
|
| Application: | WB | |
| Species: | human | |
| Author: | Han Jiang | |
| Pubmed ID: | 25329616 | |
| Journal: |
Int J Mol Sci
|
|
| Title: |
Association between the Hypomethylation of Osteopontin and Integrin β3 Promoters and Vascular Smooth Muscle Cell Phenotype Switching in Great Saphenous Varicose Veins.
|
|
| Application: | IHC | |
| Species: | human | |
| Author: | Kai Qu | |
| Pubmed ID: | 25641377 | |
| Journal: |
J Gastroenterol Hepatol
|
|
| Title: |
Transarterial chemoembolization aggravated peritumoral fibrosis via HIF-1α dependent pathway in hepatocellular carcinoma.
|
|
| Application: | WB, IHC | |
| Species: | human, rat | |
| Author: | Ci-Hang Bao | |
| Pubmed ID: | 25677618 | |
| Journal: |
Biochem Biophys Res Commun
|
|
| Title: |
Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma.
|
|
| Application: | WB, IHC | |
| Species: | human | |
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文献和实验相关实验
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠平滑肌抗体 (SMA)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 SMA 单抗包被于酶标板上，标准品和样品中的 SMA 与单抗结合，加入生物素化的抗大鼠 SMA ，形成免疫复合物连接在板上，辣根
上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
多是多克隆；而小鼠来源的抗体多是单克隆，但也有另外。这条主要要与后面的二抗来源相匹配。（3）实验目的是检测什么种属的抗原，即species reactivity。这一点很重要，一般说明书上都有注明，如小鼠Ms、大鼠Rat、人Hum等等。（4）能否做免疫组化。一般一抗说明书都会注明做WB、IHC、ICC、IF等，建议最好选择注明的抗体，因为一般都是经过文章证明。（5）检测标本类型。用于检测石蜡切片还是冰冻切片，一般能做石蜡切片的抗体，可能都可以用来检测冰冻切片，但能做冰冻切片的抗体，不一定能检测石蜡
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SMA免疫组化抗体
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