- ¥1200
- affinty
- DF6090
- 中国
- 2025年11月07日
- WB,IHC,IF/ICC,ELISA
- 兔
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
p27 Antibody
- 供应商：
上海研卉生物科技有限公司
- 宿主：
兔
- 保质期：
一年
- 应用范围：
WB,IHC,IF/ICC,ELISA
- 适应物种：
Human, Mouse, Rat
- 抗原来源：
其它
- 库存：
大量
- 是否单克隆：
多克隆
- 抗体英文名：
p27 Antibody
- 规格：
50ul
名 称:
p27 抗体
货 号:
DF6090
来 源:
Rabbit
应 用:
WB,IHC,IF/ICC,ELISA
反 应:
Human, Mouse, Rat
预 测:
Bovine(86%), Sheep(86%), Rabbit(100%), Dog(100%), Chicken(86%)
蛋白号:
P46527
分子量:
27kDa
浓 度:
1mg/ml
Alternative Names:Expand▼
AA408329; AI843786; Cdki1b; CDKN 1B; CDKN 4; CDKN1B; CDKN4; CDN1B_HUMAN; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; KIP 1; KIP1; MEN1B; MEN4; OTTHUMP00000195098; OTTHUMP00000195099; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor; p27Kip1;
Applications:
WB 1:200-1:500, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Reactivity:
Human, Mouse, Rat
Predicted Reactivity:
Bovine(86%), Sheep(86%), Rabbit(100%), Dog(100%), Chicken(86%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Specificity:
p27 Antibody detects endogenous levels of total p27.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Mouse p27 PCR Using Gitschier Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGTGCCATCTCTAT) produce a 0.5kB PCR product. Wildtype allele: mgK-3 (above) plus mcK-5 (GAGCAGACGCCCAAGAAGC) produce ~1kB product. Stock Solutions
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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