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文献和实验Green lab protocol for vacuum infiltration transformation of Arabidopsis
inside a vacuum desiccator. Invert pots containing plants to be infiltrated into the solution so that the entire plant is covered, including rosette, but not too much of the soil is submerged. One good way to do this is to place the corners of the pots on rubber
E-Z 96 X-press Plasmid Vacuum Protocol
with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate
Green lab protocol for vacuum infiltration transformation of Arabidopsis
catalog # vis-01) 5. Place resuspended culture in a Rubbermaid container inside a vacuum desiccator. Invert pots containing plants to be infiltrated into the solution so that the entire plant is covered
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