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- WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
凝血酶(凝血因子II)重链抗体规格
- 抗体英文名：
Anti-Thrombin heavy chain
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海博湖
- 库存：
大量
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Thrombin heavy chain
- 规格：
0.2ml/200μg
英文名称 Anti-Thrombin heavy chain
中文名称 凝血酶(凝血因子II)重链抗体规格
别 名 coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II (thrombin); Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin; Thrombin heavy chain.
凝血酶(凝血因子II)重链抗体规格浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Fish
产品类型 一抗
研究领域 心血管 细胞生物 激酶和磷酸酶 合成与降解
蛋白分子量 predicted molecular weight: 28/68kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Thrombin heavy chain
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.02% Sodium azide
凝血酶(凝血因子II)重链抗体规格产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.
Function : Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Gamma Thrombin is a proteolyzed form of Alpha Thrombin. Gamma Thrombin consists of four chains (A, B1, B5, B4) with a disulfide link between the A peptide and the B5 peptide.
Subunit : Heterodimer (named alpha-thrombin) of a light and a heavy chain; disulfide-linked. Forms a heterodimer with SERPINA5.
Subcellular Location : Secreted, extracellular space.
Tissue Specificity : Expressed by the liver and secreted in plasma.
Post-translational modifications : The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
DISEASE : Factor II deficiency (FA2D) [MIM:613679]: A very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Note=The disease is caused by mutations affecting the gene represented in this entry.
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Thrombophilia due to thrombin defect (THPH1) [MIM:188050]: A multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=The disease is caused by mutations affecting the gene represented in this entry. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
Pregnancy loss, recurrent, 2 (RPRGL2) [MIM:614390]: A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Similarity : Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
Database links : UniProtKB/Swiss-Prot: P00734.2
凝血酶(凝血因子II)重链抗体规格抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
凝血酶(凝血因子II)重链抗体规格实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
L型电压依赖型通道β4抗体
电压依赖性通道CACNA1G+CACNA1H抗体
结合蛋白1抗体
神经型合成酶结合蛋白抗体
毒蕈碱型乙酰胆碱受体M4抗体
脑肿瘤抑癌蛋白1抗体 Anti-Tomoregulin-1 WB IHC-P IHC-F IF 100ul
肺癌抑癌蛋白1抗体 Anti-TUSC1 WB IHC-P IHC-F IF 100ul
前列腺癌抑癌蛋白3抗体 Anti-TUSC3 WB IHC-P IHC-F IF 100ul
甲状腺激素受体α1抗体 Anti-THRA1 WB IHC-P IHC-F IF 100ul
甲状腺激素受体α1+α2抗体 Anti-THRA1+2 WB IHC-P IHC-F IF 1
趋化素抗体 Anti-chemerin WB IHC-P IHC-F IF 100ul
5号染色体开放阅读框55抗体 Anti-C5orf55 WB IHC-P IHC-F IF 100ul
细胞表面趋化因子受体7抗体 Anti-CXCR7 WB IHC-P IHC-F IF 100ul
细胞色素c氧化酶7A2抗体 Anti-COX7A2 WB IHC-P IHC-F IF 100ul
7号染色体开放阅读框16抗体 Anti-C7orf16 WB IHC-P IHC-F IF 100ul
録霉素麦芽提取物琼脂培养基250g用于真菌的分离培养
TPY琼脂培养基 250(g) incubation media TPY琼脂培养基 250(g)
DME培养基 DMEM 10升 高糖，含酸和谷酰胺
牛津琼脂(OXA)基础250用于单增李氏菌的选择性分离incubationmedia牛津琼脂(OXA)基础250用于单增李氏菌的选择性分离
菌种芽孢培养基 250g 用于嗜热脂肪芽孢杆菌芽孢培养
AKAgar(No.2)
Fraser肉汤基础 与Fraser肉汤添加物合用选择增强与鉴定李斯特菌 500g incubation media Fraser肉汤基础 与Fraser肉汤添加物合用选择增强与鉴定李斯特菌 500g
扁柄侧耳 食用 支/瓶
LBth
YeastPeptoneDextroseAgar
凝血酶(凝血因子II)重链抗体规格麦迪、麦白霉素检定培养基(普通轻质斜面培养基)250g麦迪(白)霉素效价测定
酪胨琼脂 250(g) incubation media 酪胨琼脂 250(g)
改良马丁培养基(真菌培养基) 250g 用于真菌培养及药品和生物制品无菌检查用。
肝浸粉养基原材料，为营养要求高的细菌提供生长营养成份incubationmedia肝浸粉养基原材料，为营养要求高的细菌提供生长营养成份
明胶盐缓冲液 250g 用于检测肉毒梭菌时样品稀释液
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文献和实验相关实验
功能，无决定性的影响，不再列入凝血因子的编号。因子 VI 事实上是活化的第五因子，已经取消因子VI的命名。 凝血过程 可分为凝血酶原酶复合物(也称为凝血酶原激活复合物)的行程、凝血酶原的激活和纤维蛋白的生成三个基本步骤。 凝血功能检查 可以在术前了解患者有无凝血功能的异常，有效防止在术中及术后出现出血不止等意外情况，从而获得最佳的手术效果。 机体的止血功能是由血小板、凝血系统、纤溶系统和血管内皮系统等的共同作用来完成的。过去我们曾用出血时间作为止血功能缺陷
主要凝血因子： 因子I，纤维蛋白原 因子II，凝血酶原 因子III，组织因子 因子IV，钙因子（Ca2+） 因子V，促凝血球蛋白原，易变因子 因子VII，转变加速因子前体，促凝血酶原激酶原，辅助促凝血酶原激酶 因子VIII，抗血友病球蛋白A（AHGA），抗血友病因子A（AHFA），血小板辅助因子I，血友病因子VIII或A， 因子IX，抗血友病球蛋白B（AHGB），抗血友病因子B（AHFB），血友病因子IX或B 因子X
）。它们的共同特点是通过接触反应启动内源凝血途径医学教育网搜集|整理，并与激肽、纤溶和补体等系统相联系。 （3）对凝血酶敏感的凝血因子： 包括FⅠ、FⅤ、FⅧ和FⅩⅢ，它们的共同特点是对凝血酶敏感。 （4）其他因子： 包括FⅢ、FⅣ。正常情况下，FⅢ不存在于血液中。FⅣ即Ca离子。
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凝血酶(凝血因子II)重链抗体规格
询价
