Rat Carboxylesterase 1 / CES1

Rat Carboxylesterase 1 / CES1 ELISA Kit (Sandwich ELISA) - LS-F7737

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LS-F7737-1
1 plate
$1,065 USD
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Product Description

LS-F7737 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Carboxylesterase 1 / CES1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Carboxylesterase 1 / CES1 as low as 14.1 picograms per milliliter.
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Carboxylesterase 1 / CES1
Synonyms
 
CES1, ACAT, Brain carboxylesterase hBr1, Carboxylesterase 1, Carboxylesterase 2 (liver), CEH, CES1A1, CES1A2, Cocaine carboxylesterase, Egasyn, HMSE, Liver carboxylesterase 1, HCE-1, PCE-1, Retinyl ester hydrolase, TGH, Triacylglycerol hydrolase, REH, SES1, CE-1, Cholesteryl ester hydrolase, HMSE1, Serine esterase 1
Reactivity
 
Rat
Specificity
 
Carboxylesterase 1 (CES1) No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogs was observed.
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
31.25 - 2000 pg/ml
Sensitivity
 
14.1 pg/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Adhesive Plate Sealers
• Standard (Lyophilized)
• Sample Diluent
• Detection Reagent A (Green)
• Detection Reagent B (Red)
• Assay Diluent A
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30x concentrate)

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About Carboxylesterase 1 / CES1
 
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.Uniprot: P23141 NCBI: L07764 AAA16036.1

 

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