Zymo-Spin™ ChIP Kit

Active Motifs ChIP-IT Kit

that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP

ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit

were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated

MicroElute DNA Clean-Up Kit

the collection tubes for next step.    6) Add 700 ul of SPW Wash buffer diluted with ethanol to the HiBind™ MicroElute™ DNA column and spin at 10,000 x g for 1 minute. Discard the flow-through and place the column back into same 2mL collection tube.