Femto Bacterial DNA Quantifica

Mag-Bind Soil DNA Kit Protoco

, should be used.   2. Add 80 ul Buffer DS and vortex to mix.   3. Incubate at 70°C for 10 min. Briefly vortex the tube once during the incubation. For some difficult lysis bacterial, Increase the temperature to 90°C.   4. Centrifuge at 13,000 x g

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.

Quantification made easy

determination DNA, RNA, oligonucleotides and even mononucleotides can be measured directly in aqueous solutions in a diluted or undiluted form. Aqueous buffers with low ion concentrations (e.g. TE buffer) are ideal for this method. The concentration