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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 英文名:
Recombinant Human TNF RII/TNFRSF1B/Fc Chimera, CF
- 规格:
50 ug
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罗氏原料,请来电来邮咨询。
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文献和实验Production and Characterization of Recombinant Human and Murine TNF
takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity
(9 ). Because its systemic administration was shown to mediate the regression of some mouse tumors (10 ), TNF-α has attracted much attention as a potential antitumor reagent (11 ). However, the problem of its dose-dependent toxicity has been particularly apparent in human
cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
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