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- 详细信息
- 技术资料
- 英文名:
jetPEI®-Hepatocyte For primary hepatocytes
- 规格:
0.5ml
原代肝细胞的高效转染(在肝细胞样细胞和原代肝细胞中的转染效率高达50%)
- Efficient transfection of primary hepatocytes (up to 50% of transfection efficiency in hepatocyte-like cells and primary hepatocytes)
- Gentle to the cells
- Easy-to-use protocol
- Compatible with serum and antibiotics
Specifications
| Reagent | jetPEI®-Hepatocyte |
|---|---|
| Molecule delivered | DNA |
| Applications | DNA transfection of primary hepatocytes |
| Cell types | Primary hepatocytes |
| Number of transfections | 0.5 ml of jetPEI®-Hepatocyte transfection reagent is sufficient to perform up to 150 transfections in 24-well plates or 30 transfections in 60-mm plates |
| Storage | 5°C ± 3°C, for up to 12 months |
| Provided with | 150 mM NaCl |
Ordering information
| Reference Number | Amount of reagent | Amount of NaCl |
|---|---|---|
| 102-05N | 0.5 ml | 50 ml |
Ma, J., Huang, C., Yao, X., Shi, C., Sun, L., Yuan, L., Lei, P., Zhu, H., Liu, H., Wu, X., Ning, Q., Zhou, C., Shen, G. (2012). Inhibition of Hepatitis B Virus and Induction of Hepatoma Cell Apoptosis by ASGPR-Directed Delivery of shRNAs., PLoS One 7, e4609.
Andrieux, L. O., Fautrel, A., Bessard, A., Guillouzo, A., Baffet, G., Langouet, S. (2007). GATA-1 is essential in EGF-mediated induction of nucleotide excision repair activity and ERCC1 expression through ERK2 in human hepatoma cells., Cancer Res 67, 2114.
Naimi, M., Gautier, N., Chaussade, C., Valverde, A. M., Accili, D., Van Obberghen, E. (2007). Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes., Endocrinology 148, 2424.
Ben-Shlomo, S., Zvibel, I., Shnell, M., Shlomai, A., Chepurko, E., Halpern, Z., Barzilai, N., Oren, R., Fishman, S. (2011). Glucagon-like peptide-1 reduces hepatic lipogenesis via activation of AMP-activated protein kinase., J Hepatol 54, 1214.
Baltrusch, S., Francini, F., Lenzen, S., Tiedge, M. (2005). Interaction of glucokinase with the liver regulatory protein is conferred by leucine-asparagine motifs of the enzyme., Diabetes 54, 2829.
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