肝细胞DNA转染试剂jetPEI®-Hepatocyte For primary hepatocytes

肝细胞DNA转染试剂jetPEI®-Hepatocyte F

or primary hepatocytes
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  • ¥4959
  • 法国polyplus
  • 102-05N
  • 2025年07月08日
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    • 详细信息
    • 技术资料
    • 英文名

      jetPEI®-Hepatocyte For primary hepatocytes

    • 规格

      0.5ml

    肝细胞DNA转染试剂jetPEI®-Hepatocyte For primary hepatocytes
    原代肝细胞的高效转染(在肝细胞样细胞和原代肝细胞中的转染效率高达50%)
    • Efficient transfection of primary hepatocytes (up to 50% of transfection efficiency in hepatocyte-like cells and primary hepatocytes)
    • Gentle to the cells
    • Easy-to-use protocol
    • Compatible with serum and antibiotics

    Specifications

    Reagent

    jetPEI®-Hepatocyte

    Molecule delivered

    DNA

    Applications

    DNA transfection of primary hepatocytes

    Cell types

    Primary hepatocytes

    Number of transfections

    0.5 ml of jetPEI®-Hepatocyte transfection reagent is sufficient to perform up to 150 transfections in 24-well plates or 30 transfections in 60-mm plates

    Storage

    5°C ± 3°C, for up to 12 months

    Provided with

    150 mM NaCl

    Ordering information

    Reference Number Amount of reagent Amount of NaCl
    102-05N 0.5 ml 50 ml

    0.5 ml of jetPEI®-Hepatocyte transfection reagent is sufficient to perform ca. 150 transfections in 24-well plates.

    文献:

    Ma, J., Huang, C., Yao, X., Shi, C., Sun, L., Yuan, L., Lei, P., Zhu, H., Liu, H., Wu, X., Ning, Q., Zhou, C., Shen, G. (2012). Inhibition of Hepatitis B Virus and Induction of Hepatoma Cell Apoptosis by ASGPR-Directed Delivery of shRNAs., PLoS One 7, e4609.

    Andrieux, L. O., Fautrel, A., Bessard, A., Guillouzo, A., Baffet, G., Langouet, S. (2007). GATA-1 is essential in EGF-mediated induction of nucleotide excision repair activity and ERCC1 expression through ERK2 in human hepatoma cells., Cancer Res 67, 2114.

    Naimi, M., Gautier, N., Chaussade, C., Valverde, A. M., Accili, D., Van Obberghen, E. (2007). Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes., Endocrinology 148, 2424.

    Ben-Shlomo, S., Zvibel, I., Shnell, M., Shlomai, A., Chepurko, E., Halpern, Z., Barzilai, N., Oren, R., Fishman, S. (2011). Glucagon-like peptide-1 reduces hepatic lipogenesis via activation of AMP-activated protein kinase., J Hepatol 54, 1214.

    Baltrusch, S., Francini, F., Lenzen, S., Tiedge, M. (2005). Interaction of glucokinase with the liver regulatory protein is conferred by leucine-asparagine motifs of the enzyme., Diabetes 54, 2829.

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