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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
上海烜雅生物科技有限公司
- 肿瘤类型:
人高转移肺癌细胞(STR鉴定)
- 细胞类型:
科研
- ATCC Number:
/
- 品系:
人高转移肺癌细胞(STR鉴定)
- 组织来源:
人高转移肺癌细胞(STR鉴定)
- 相关疾病:
人高转移肺癌细胞(STR鉴定)
- 物种来源:
鼠/人/其它
- 免疫类型:
人高转移肺癌细胞(STR鉴定)
- 细胞形态:
上皮细胞样,贴壁生长
- 是否是肿瘤细胞:
/
- 器官来源:
人高转移肺癌细胞(STR鉴定)
- 运输方式:
常温运输/干冰运输
- 年限:
永久
- 生长状态:
贴壁/悬浮
- 英文名:
95-D
- 规格:
1x10^6
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:



(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人高转移肺癌细胞(STR鉴定)相关产品:
| Acc-2 | 涎腺腺样囊性癌 |
| 786-O | 肾透明细胞腺癌 |
| Acc-3 | 涎腺腺样囊性癌 |
| PC-3 | 前列腺癌 |
| KB | 口腔表皮样癌 |
| T-24 | 膀胱变移细胞癌 |
| HEp-2 | 喉表皮样癌 |
| SCaBER | 膀胱鳞癌 |
| CNE | 鼻咽癌 |
| Ho-8910 | 卵巢癌 |
| TT | 髓性甲状腺癌 |
| L1 | 卵巢癌 |
| Bcap-37 | 乳腺癌 |
| HeLa | 宫颈癌 |
| ZR-75-30 | 乳腺癌 |
| HeLa229 | 宫颈癌 |
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文献和实验据统计约 30% 细胞系被交叉污染或错误辨识,因使用了交叉污染或错误辨识的细胞而导致研究结论错误、结果不可重复、临床细胞治疗灾难性后果……,这浪费大量时间、精力和金钱。Everything was going along fine until they discovered their HeLa cell line expressed Y chromosome markers因此近年 NIH、ATCC、Nature 和 Science 等对此多次发出呼吁,要求研究者对细胞进行鉴定。STR 基因
人头颈部肿瘤AM 人腺样囊性癌细胞(高转移)A2 人腺样囊性癌细胞A83 人腺样囊性癌细胞Hep-2 人喉表皮癌细胞KB 人口腔上皮癌细胞CNE-2Z 人鼻咽癌母系细胞肺癌A549 人肺腺癌细胞801-D 人巨细胞性肺癌细胞肺793 肺腺癌细胞H125 人肺癌细胞NCI-H460 人肺癌细胞LTEP-Sm1 人小细胞肺癌细胞(SCLC)消化系统肿瘤 HT-29 人结肠癌细胞 PC-3 人胰腺癌细胞 SW480 人结肠
cypress1975 最近有个实验要做,但是不知道用哪种方法较好。 实验目的:检测80个已知其序列的基因在肺癌细胞系的肿瘤干细胞和非肿瘤干细胞中的表达差异。 用哪种方法比较可靠,而且省钱,大约需要多少经费和时间。 下面这些方法那些可以用?有没有其他方法? 1. 基因表达指纹( gene expression fingerprinting, gEF. 2.基因表达系统分析( serial









