- ￥2570
- PEPROTECH
- 500-P10Bt-25
- 进口
- 2025年07月16日
- WB elisa
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
500-P10Bt-25
- 靶点：
生物素标记VEGF165抗体
- 目录编号：
500-P10Bt-25
- 抗体英文名：
Biotinylated Rabbit Anti-Human VEGF165
- 抗体名：
生物素标记VEGF165抗体
- 适应物种：
人
- 应用范围：
WB elisa
- 浓度：
Biotinylated Rabbit Anti-Human VEGF165
- 保存条件：
低温
- 规格：
25ug
产品详情
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVEGF. Anti-Human VEGF specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli-derived, 38.2 kDa Recombinant Human VEGF165 (PeproTech catalog# 100-20)
Sandwich ELISA: To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human VEGF (500-P10) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hVEGF.
Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
| 500-P10-50 | Rabbit Anti-Human VEGF165 | 50ug |
| 500-P10-100 | Rabbit Anti-Human VEGF165 | 100ug |
| 500-P10-1000 | Rabbit Anti-Human VEGF165 | 1000ug |
| 500-P10Bt-25 | Biotinylated Rabbit Anti-Human VEGF165 | 25ug |
| 500-P10Bt-50 | Biotinylated Rabbit Anti-Human VEGF165 | 50ug |
| 500-P10Bt-1000 | Biotinylated Rabbit Anti-Human VEGF165 | 1000ug |
| 500-P10G-50 | Goat Anti-Human VEGF165 | 50ug |
| 500-P10G-100 | Goat Anti-Human VEGF165 | 100ug |
| 500-P10G-1000 | Goat Anti-Human VEGF165 | 1000ug |
| 500-P10GBt-25 | Biotinylated Goat Anti-Human VEGF165 | 25ug |
| 500-P10GBt-50 | Biotinylated Goat Anti-Human VEGF165 | 50ug |
| 500-P10GBt-1000 | Biotinylated Goat Anti-Human VEGF165 | 1000ug |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
位。 IF（免疫荧光检测）是采用荧光素标记物作为探针，形成的抗原抗体复合物上带有荧光素，在荧光显微镜下，受激发光的照射后发出一定波长的荧光，从而对细胞、组织中的抗原进行定位或定量。 表 1 IHC/ICC与IF特点对比 IHC/ICC 与 IF 染色中使用的抗体主要为一抗与二抗，根据是否选用二抗以及二抗标记物类型可形成多种不同的检测系统。信号放大是免疫组化实验中的重要步骤，信号放大可以使目标蛋白质的信号从微弱到明显，从而更容易被观察和定量。现有的信号放大技术多为链霉亲和素-生物素法，该方法基于链酶亲和
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