6x-His Tag Monoclonal Antibody

6x-His Tag Monoclonal Antibody (3D5)

货号 R930-25

50 µL

产品详细信息

NOTE: R930-25 was previously sold under product #46-0693. When re-ordering, please use #R930-25.

This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells.

This product contains enough material for 25 Western blots. It is functionally tested using 500 ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. A signal can be detected when tested against 100 ng of recombinant Positope™ protein. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).

This product has also been used successfully to immunoprecipitate fusion proteins that contain the 6x His tag.

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