【ER-a】重组人雌激素受体α

【ER-a】重组人雌激素受体α
重组人Estrogen Receptor alpha蛋白(ab153776)

产品名称
重组人Estrogen Receptor alpha蛋白
参阅全部 Estrogen Receptor alpha 蛋白酶
蛋白长度
Protein fragment


性质
Recombinant
来源
Escherichia coli
氨基酸序列
Accession
P03372
种属
Human
序列
MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPA VYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPL NSVSPSPLMLLHPPPQ
分子量
12 kDa
氨基酸
1 to 116
标签
His tag C-Terminus



Our Abpromise guarantee covers the use of ab153776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

SDS-PAGE

HPLC
内毒素水平
< 1.000 Eu/µg
纯度
> 95 % SDS-PAGE.
ab153776 is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. It is lyophilized from an 0.2 µM filtered solution.
形式
Lyophilised
浓度
由溶解方法决定


稳定性和存储

Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

pH: 7.40
Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
复溶
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 µg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


别名

DKFZp686N23123
ER
ER alpha

see all
功能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
翻译后修饰
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
细胞定位
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

Target information above from: UniProt accession P03372
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

This product has been referenced in:

Yin JJ et al. Controllable drug uptake and nongenomic response through estrogen-anchored cyclodextrin drug complex. Int J Nanomedicine 10:4717-30 (2015). Read more (PubMed: 26251594) »