FITC抗体标记试剂盒， Tags & Cell Marke
rs Epitope Tags Conjugates FITC荧光偶联试剂盒(Fast) - Lightning-Link- ￥3166
- ABCAM
- ab188285
- 2025年07月22日
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
FITC荧光偶联试剂盒(Fast) - Lightning-Link
- 抗体英文名：
FITC抗体标记试剂盒
- 抗体名：
FITC荧光偶联试剂盒(Fast) - Lightning-Link
- 标记物：
FITC
- 浓度：
FITC抗体标记试剂盒
- 规格：
100ug
货号(ab188285)
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说明
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Fluorescein Labeling Kit. 310-0005 is the same as the 100 ug size. 310-0010 is the same as the 3 x 100 ug size. 310-0030 is the same as the 3 x 10 ug size. 310-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to FITC
Kit size Recommended
amount of antibody1Maximum
amount of antibodyMaximum antibody
volume23 x 10 µg 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 1 x 100 µg 1 x 200 µg 1 x 100µL 3 x 100 µg 3 x 100 µg 3 x 200 µg 3 x 100 µL 1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL 1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose 1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 mg 100 µg 3 x 10 µg 3 x 100 µg ab273993 - FITC Mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg ab273994 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl ab273995 - Quencher reagent猝灭剂 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
phosphate‐buffered saline (DPBS; with CaCl 2 and MgCl 2 ; e.g., Invitrogen) Cell lysis buffer: 50 mM Tris·Cl, pH 7.4 ( appendix 2E )/150 mM NaCl/1% (v/v) Triton X‐100/1× protease inhibitors
of 46 chromosomes, 22 pairs of autosomes, and 1 pair of sex chromosomes (the X and Y chromosomes). B BAC: See bacterial artificial chromosome. Bacterial artificial chromosome (BAC): A vector used to clone DNA fragments (100- to 300-kb insert size
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
have been isolatedfrom whole tissue or cell culture (5 , 6) , relatively small quantitieshave been obtained in this manner. There are no reportsof recombinant expression in prokaryotic systems, presumablybecause of the folding problems associated with production
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