FITC抗体标记试剂盒， Tags & Cell Marke

货号(ab188285)

• 说明

  This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link^® Rapid Fluorescein Labeling Kit. 310-0005 is the same as the 100 ug size. 310-0010 is the same as the 3 x 100 ug size. 310-0030 is the same as the 3 x 10 ug size. 310-0015 is the same as the 1 mg size.

  Amount and volume of antibody for conjugation to FITC

  Kit size	Recommended amount of antibody1	Maximum amount of antibody	Maximum antibody volume2
  3 x 10 µg	3 x 10 µg	3 x 20 µg	3 x 10 µL
  100 µg	1 x 100 µg	1 x 200 µg	1 x 100µL
  3 x 100 µg	3 x 100 µg	3 x 200 µg	3 x 100 µL
  1 mg	1 x 1 mg	1 x 2 mg	1 x 1 mL

  ¹ Using the maximum amount of antibody may result in less labelling per antibody.

  ² Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

  Buffer Requirements for Conjugation

  Buffer should be pH 6.5-8.5.

  Compatible buffer constituents
  If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

  50mM / 0.6% Tris1	0.1% BSA2	50% glycerol
  0.1% sodium azide	PBS	Potassium phosphate
  Sodium chloride	HEPES	Sucrose
  Sodium citrate	EDTA	Trehalose

  ¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%
  ² BSA can also interfere with the use of the conjugated antibody in tissue staining.
  

  Incompatible buffer constituents

  Thiomerosal	Proclin	Glycine
  Arginine	Glutathione	DTT

  If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

  Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  Storing and handling conjugation kits

  Lyophilized Lightning-Link^® components are hygroscopic.

  Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

• 存放说明

  Store at -20°C. Please refer to protocols.

• 组件	1 mg	100 µg	3 x 10 µg	3 x 100 µg
  ab273993 - FITC Mix	1 x 1mg	1 x 100µg	3 x 10µg	3 x 100µg
  ab273994 - Modifier reagent	1 x 200µl	1 x 200µl	1 x 200µl	1 x 200µl
  ab273995 - Quencher reagent猝灭剂	1 x 200µl	1 x 200µl	1 x 200µl	1 x 200µl