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- 2025年07月22日
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- 库存：
ab102921
- 靶点：
链霉亲和素抗体标记试剂盒
- 目录编号：
ab102921
- 抗体英文名：
Streptavidin偶联试剂盒 - Lightning-Link
- 抗体名：
链霉亲和素抗体标记试剂盒
- 标记物：
Streptavidin
- 浓度：
Streptavidin偶联试剂盒 - Lightning-Link
- 规格：
100ug
Streptavidin偶联试剂盒 - Lightning-Link® (ab102921)
说明
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Streptavidin Labeling Kit. 708-0005 is the same as the 100 µg size. 708-0010 is the same as the 3 x 100 ug size. 708-0030 is the same as the 3 x 10 ug size. 708-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Streptavidin
| Kit size | Recommended maximum amount of antibody |
Maximum antibody volume1 |
| 3 x 10 µg | 3 x 10 µg | 3 x 10 µL |
| 100 µg | 100 µg | 100 µL |
| 3 x 100 µg | 3 x 100 µg | 3 x 100 µL |
| 1 x 1 mg | 1 x 1 mg | 1 x 1 mL |
1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine | ||
| Arginine | Glutathione | DTT |
||
| 组件 | 1 mg | 100 µg | 3 x 10 µg | 3 x 100 µg |
|---|---|---|---|---|
| ab274106 - Modifier reagent | 1 x 200µl | 1 x 200µl | 1 x 200µl | 1 x 200µl |
| ab274296 - Quencher reagent | 1 x 200µl | 1 x 200µl | 1 x 200µl | 1 x 200µl |
| ab274136 - Streptavidin mix | 1 x 1mg | 1 x 100µg | 3 x 10µg | 3 x 100µg |
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Related Products
- Antibody Concentration And Clean-Up Kit (ab102778)
- Antibody Purification Kit (Protein A) (ab102784)
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