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- 详细信息
- 文献和实验
- 技术资料
- 库存:
NBP3-12091-0.025mg
- 靶点:
Complement C4c Antibody (99-72-18) - BSA Free
- 级别:
1 mg/ml
- 目录编号:
NBP3-12091-0.025mg
- 克隆性:
单克隆
- 抗体英文名:
Complement C4c Antibody
- 抗体名:
Complement C4c Antibody (99-72-18) - BSA Free
- 标记物:
可以提供AF488等标记
- 宿主:
Mouse
- 适应物种:
人
- 应用范围:
WB, ELISA, ICC/IF, IHC, IP
- 浓度:
Complement C4c Antibody
- 规格:
0.025mg
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone 99-72-18
Clonality Monoclonal
Host Mouse
Conjugate Unconjugated
Format BSA Free
Concentration 1 mg/ml
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Complement C4c Antibody (99-72-18) - BSA Free Summary
| Immunogen |
Synthetic peptide consisting of the last 15C-terminal residues of the C4c a4-domain (Cys-EELVYELNPLDHRGR).
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| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Protein A or G purified
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| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Packaging, Storage & Formulations
Alternate Names for Complement C4c Antibody (99-72-18) - BSA Free
Background
C4c is a compoment in the Complement cascade and is a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c (soluble). C4c consists of a g-chain (30 kDa), a b-chain (75 kDa), and two a-domains denoted a3 (27 kDa) and a4 (14 kDa). C4c reacts with C1 and C2 to form C3 convertase of the classic activation pathway.
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
to free 3’-OH DNA ends in a template-independent manner, is used to label DNA strand breaks. Incorporated nucleotides are detected by a secondary antibody, conjugated with peroxidase. After substrate reaction, stained cells can be detected under light
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
. Meanwhile in Coplin jars: 9. Hydrate: 2x SSC x2-5 min. 10. (Optional, to reduce cytoplasm bkgd: 10 mg/mL RNAse (DNAse-free) in 2x SSC, 37ºC x30 min.) 11. Denature in 2x SSC + Formamide (50% vol), 72ºC x2-5 min. 12. Dehydrate: Ice cold 70% EtOH, 85% EtOH, 95
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