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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海研卉
- 库存:
AB188288
- 靶点:
CY5抗体标记试剂盒
- 目录编号:
AB188288
- 抗体英文名:
Cy5®偶联试剂盒(Fast) - Lightning-Link®
- 抗体名:
CY5抗体标记试剂盒
- 标记物:
CY5
- 浓度:
Cy5®偶联试剂盒(Fast) - Lightning-Link®
- 规格:
100UG
Cy5®偶联试剂盒(Fast) - Lightning-Link®
说明
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Cy5 Labeling Kit. 342-0005 is the same as the 100 µg size. 342-0010 is the same as the 3 x 100 ug size. 342-0030 is the same as the 3 x 10 ug size. 342-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Cy5
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 µg | 3 x 10 µg | 3 x 20 µg | 3 x 10 µL |
| 100 µg | 1 x 100 µg | 1 x 200 µg | 1 x 100 µL |
| 3 x 100 µg | 3 x 100 µg | 3 x 200 µg | 3 x 100 µL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
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文献和实验生物技术有限公司自2007年开始以结核抗体基因检测技术进行结核试剂盒开发研究,并以金标免疫层析阵列技术检测结核病人的血清抗体,从技术层面解决了结核分枝杆菌抗体检测特异性不高的问题,而且还利用两种抗原检测的互补效果提高了检测灵敏度。目前,成功研制的结核抗体快速检测(只需5-6分钟)试剂盒获得新器械产品注册证后已进入市场销售,为结核抗体检测提供了一项新的快速检测手段。
直标法因为Cy5标记效率低而导致Cy3、Cy5两种颜色信号强度不同的现象。试剂盒提供除荧光染料外的全部试剂,足够作10次标记。 6)Vysis公司的Microarray Nick Translation Kit 是配合Vysis公司的Genomic Microarray--AmpliOnc I Microarray的荧光标记试剂盒,采用缺口平移的方法将荧光标记的dUTP掺入纯化的样品基因组DNA中作为探针,和AmpliOnc I Microarray进行杂交。 除了厂家为自家的DNA
可以将干扰扣除,但补偿值越大,补偿后的信号分辨率会降低越厉害。最好选择很少或者没有重叠的荧光基团,以最大限度减少这种渗漏。不同激光激发的染料是首选,如PE和APC,但在某些情况下,这可能与尽量选择亮的荧光素的规则是冲突的。如在进行四色分析时,经常会将PE-Cy5标记的抗体与APC标记的抗体搭配使用。因为Cy5的激发波长与APC的激发波长相近,所以PE-Cy5与APC均可被红色激光(640nm)所激发,而两者的发送光谱也比较接近,导致PE-Cy5与APC的颜色补偿很难调整。相反,由于PE-Cy5.5中的
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