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- 详细信息
- 询价记录
- 技术资料
- 抗体名：
大鼠CD3功能性抗体
- 亚型：
G4.18
- 保质期：
一年
- 抗原来源：
其它
- 是否单克隆：
多克隆
- 抗体英文名：
大鼠CD3功能性抗体
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 规格：
500ug
Additional Information
|
Clone:
|
G4.18
|
|
Format:
|
SAFIRE Purified
|
|
Applications:
|
FC, IHC, FA
|
|
Reactivity:
|
Rat
|
|
Isotype:
|
Mouse IgG3, kappa
|
| Research Interest: | Adaptive Immunity |
| Cell Type: | NK and NKT Cells |
|
Preparation:
|
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
|
|
Formulation:
|
Phosphate-buffered aqueous solution, ph7.2.
|
|
References:
|
Nicolls, M. R., Aversa, G. G., Pearce, N. W., Spinelli, A., Berger, M. F., Gurley, K. E., & Hall, B. M. (1993). Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.Transplantation, 55(3), 459-468. Morris, D. L., & Komocsar, W. J. (1997). Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats.Journal of pharmacological and toxicological methods, 37(1), 37-46. Tran, G. T., Carter, N., He, X. Y., Spicer, T. S., Plain, K. M., Nicolls, M., ... & Hodgkinson, S. J. (2001). Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on Th1 cells that is augmented by IL-4. International immunology, 13(9), 1109-1120. |
大鼠CD3功能性抗体（T细胞激活扩增）
SAFIRE纯化CD3单抗(clone: OKT3)可以与TCR复合物结合，激活T细胞
SAFIRE(Sodium Azide Free Immensely Reduced Endotoxins)类产品是BioGems品牌下 的功能性抗体产品，经特殊工艺生产、纯化，具有诸多优点，可用于免疫细胞功能的激活、 阻断、中和研究，是肿瘤免疫研究的重要工具。
产品优势
极低的内毒素含量，低于0.01EU/μg蛋白
无叠氮钠，适用于体内或体外实验
高蛋白纯度，适用于细胞治疗(CIK和CAR-T细胞制备)
该产品被引用文献：
Sensitivity to Restimulation-Induced Cell Death Is Linked to Glycolytic Metabolism in Human T Cells
https://www.jimmunol.org/content/198/1/147.short
产品信息：
| Clone | OKT3 |
| Format: | SAFIRE Purified |
| Applications | FC, FA, IHC |
| Reactivity | Human |
| Isotype: | Mouse IgG2a, kappa |
| Preperation: | The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein. |
| Formulation: | Phosphate-buffered aqueous solution, ph7.2. |
| References: | Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989. Adair, J. R., Athwal, D. S., Bodmer, M. W., Bright, S. M., Collins, A. M., Pulito, V. L., ... & Jolliffe, L. K. (1994). Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Human Antibodies, 5(1), 41-47. |
Analytical QC Report
Description: Anti-Human CD3 SAFIRE Purified (CAT# 05121-25) Lot: 25S131805121
Lane 1: Molecular weight standard
Lane 5: OKT3 reduced
FC Staining:
Cell sample: human PBMC stained with OKT3 lot# 25S131805121relevant isotype control in red.
LaL Assay Endotoxin Testing: Specification: <0.01 EU/ug
Lot# 25S131805121: 0.000995 EU/ug
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