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- Ag23549
- 2025年12月20日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
NRP1 Protein
- 抗体英文名：
NRP1 Protein
- 宿主：
兔
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 抗原来源：
其它
- 是否单克隆：
0
- 规格：
50ug
【产品介绍】Neuropilin- 1（NRP1）是细胞表面相对分子质量为103kDa的Ⅰ型跨膜糖蛋白。它有两种亚型：亚型1包括蛋白全长，有923个氨基酸组成，是结合在膜上的糖蛋 白；亚型2经过剪切后只有644个氨基酸组成，是可溶的分泌性蛋白。研究表明，NRP1通常表达在内皮细胞及一些肿瘤细胞，是 Semaphorin3A（Sema3A）和血管内皮生长因子165（vascular endothelial growth factor165，VEGF165）的受体，在神经轴突的生长、血管的生成、发育和肿瘤转移中起着重要的作用。
NRP1 Protein，NRP1蛋白
NRP1 Fusion Protein
Catalog number: Ag23549
Product Name:
human NRP1-6*His fusion protein
Catalog No:
Ag23549
Source:
e coli.-derived, PET28a
Peptide Sequence:
EWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK
(483-644 aa encoded by BC007533)
(483-644 aa encoded by BC007533)
Activity:
Not tested.
Endotoxin Level:
Please contact the lab for more information.
Note：Remove endotoxin and sterilization is required for cell assay.
Note：Remove endotoxin and sterilization is required for cell assay.
Purity:
90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Formulation:
The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
Reconstitution and Storage
Reconstitution:
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
Stability and Storage:
Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
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文献和实验相关实验
character. The N- and C- terminal and T-groups of the polypeptide can be ionized, contributing to the overall charge. The protein's net electric charge is the sum of the electric charges found on the surface of the molecule as a function of the environment
Protein Purification and Analysis
Levels of Structure in Protein Primary: A description of all covalent bonds. The sequence of AA residues Secondary: particularly stable arrangements of AA giving rise to recurring structural
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NRP1 Protein
￥1080
