蛋白激酶C zeta 抗体品牌

蛋白激酶C zeta 抗体品牌结合乙醇脱酶结构域蛋白1抗体  Anti-ZADH1  WB IHC-P IHC-F IF  100ul
指蛋白481抗体  Anti-ZBTB26  WB IHC-P IHC-F IF  100ul
指蛋白ZBTB34抗体  Anti-ZBTB34  WB IHC-P IHC-F IF  100ul
指蛋白297B抗体  Anti-ZBTB43  WB IHC-P IHC-F IF  100ul
指蛋白3抗体  Anti-ZBTB46  WB IHC-P IHC-F IF  100ul
英文名称  Anti-PKC zeta
中文名称 蛋白激酶C zeta 抗体品牌

别    名  AI098070; aPKCzeta; C80388; EC 2.7.11 .13; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta, included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r 14-3-3 ; R74924; zetaPKC; PKC-ZETA; PKC2; nPKC-zeta. 
蛋白激酶C zeta 抗体品牌浓    度  1mg/1ml
规 格  0.1ml/100μg 0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat
产品类型    一抗  
研究领域    细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶
蛋白分子量  predicted molecular weight: 66kDa
性    状  Lyophilized or Liquid
免 疫 原   KLH conjugated synthetic peptide derived from human PKC zeta
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

蛋白激酶C zeta 抗体品牌产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Function : Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukins production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In NF-kappa-B-mediated inflammatory response, can relieve the SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Is necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells.
Subunit : Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminus region).
Subcellular Location : Cytoplasm. Endosome. Cell junction. Note=In the retina, localizes in the terminals of the rod bipolar cells. Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
Post-translational modifications : CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Database links : UniProtKB/Swiss-Prot: Q05513.4


蛋白激酶C zeta 抗体品牌抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。

血小板因子4抗体  Anti-CXCL4/PF4  WB IHC-P IHC-F IF  100ul
血小板趋化因子7蛋白抗体  Anti-CXCL7/NAP-2/PPBP  WB IHC-P IHC-F IF  100ul
干扰素诱导T细胞趋化因子抗体  Anti-CXCL11  WB IHC-P IHC-F IF  100ul
B-淋巴细胞趋化因子抗体  Anti-CXCL13/BCA1  WB IHC-P IHC-F IF  100ul
凋亡加强结构域蛋白12抗体  Anti-CARD12  WB IHC-P IHC-F IF  100ul
轴突导向受体蛋白3抗体  Anti-Robo3  WB IHC-P IHC-F IF  100ul
G蛋白相关RABX5抗体  Anti-RABX5  WB IHC-P IHC-F IF  100ul
环指蛋白157抗体  Anti-RNF157  WB IHC-P IHC-F IF  100ul
核糖核酸酶抑制因子1抗体  Anti-Ribonuclease Inhibitor  WB IHC-P IHC-F IF  100ul
Notch转录调控蛋白RBPJK抗体  Anti-RBPJK  WB IHC-P IHC-F IF  100ul
叙利亚仓鼠皮肤细胞;GH-S1
人角膜细胞RNAHK miRNA5 μg
AKT1 Others Human 人 AKT1 / PKB / PKBα 杆状病毒-昆虫细胞裂解液 (阳性对照)
7721细胞，7721肝癌细胞 人肺癌细胞(淋巴结转移),NCI-H292细胞 人间充质干细胞-脂肪
人间充质干细胞-脂肪
PECAM1 Others Human 人 CD31 / PECAM1 人细胞裂解液 (阳性对照)
人胚肺成纤维细胞;WI-38 [WI38]
ST6GAL1 Others Mouse 小鼠 ST6GAL1 / CD75 人细胞裂解液 (阳性对照)
HEL(悬浮) 红白细胞白血病细胞
HEC-1-A人子宫内膜腺癌细胞 HEC-1-A human endomeial adenocarcinoma cells McCOY's 5A+10%FBS
TNFSF13B Protein Human 重组人 BLyS / TNFSF13B / BAFF 蛋白 (Fc 标签)
大鼠海马趾神经细胞(RHh)(1×106) WM451, 人黑色素瘤细胞 Human
蛋白激酶C zeta 抗体品牌叙利亚仓鼠皮肤细胞;GH-S1
人角膜细胞RNAHK miRNA5 μg
AKT1 Others Human 人 AKT1 / PKB / PKBα 杆状病毒-昆虫细胞裂解液 (阳性对照)
7721细胞，7721肝癌细胞 人肺癌细胞(淋巴结转移),NCI-H292细胞 人间充质干细胞-脂肪
人间充质干细胞-脂肪
PECAM1 Others Human 人 CD31 / PECAM1 人细胞裂解液 (阳性对照)
蛋白激酶C zeta 抗体品牌实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。