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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Mouse
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
156 - 10000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
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Popular CTSD / Cathepsin D Elisa Kits
Mouse CTSD / Cathepsin D ELISA Kit (Sandwich ELISA) - LS-F2619
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 156-10000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Mouse CTSD / Cathepsin D ELISA Kit (CLIA) - LS-F26094
Type: Quantitative Sandwich CLIA ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 2.74-2000 pg/ml
Detection:Chemiluminescent
Sample Types: Serum, Plasma
Human CTSD / Cathepsin D ELISA Kit (Sandwich ELISA) - LS-F11029
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Rat CTSD / Cathepsin D ELISA Kit (Sandwich ELISA) - LS-F7114
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 62.5-4000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Human CTSD / Cathepsin D ELISA Kit (Sandwich ELISA) - LS-F2618
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 156-10000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Product Description
LS-F24718 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CTSD / Cathepsin D in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CTSD / Cathepsin D as low as 10 picograms per milliliter.About CTSD / Cathepsin D
CTSD / Cathepsin D is a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. P07339 NM_001909 NP_001900.1
CTSD ElisaKit, CPSD ElisaKit, CLN10 ElisaKit, Lysosomal aspartyl peptidase ElisaKit, Lysosomal aspartyl protease ElisaKit, ProCathepsin D ElisaKit, CatD ElisaKit, Cathepsin D ElisaKit
More CTSD / Cathepsin D ProductsTop of Page
Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CTSD / Cathepsin D
Reactivity
Mouse
Specificity
For the detection and quantification of endogenous levels of natural and/or recombinant Mouse Cathepsin D proteins.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
156 - 10000 pg/ml
Sensitivity
10 pg/ml
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Biotinylated Detection Antibody (100x)
- Avidin-Biotin-HRP Complex (100x)
- Sample Diluent
- Biotinylated Detection Antibody Diluent
- Avidin-Biotin-HRP Complex Diluent
- Wash Buffer (20x)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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A. Formalin Fixed Cell Plates 1. Trypsinize confluent flasks 2. Pool and count cells 3. Centrifuge at 1500 rpm for 10 minutes 4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells
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