- $750
- LSBio
- 370.4 - 30000 ng/ml
- LS-F55421
- 美国
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
370.4 - 30000 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human Ischemia Modified Albumin ELISA Kit (Competitive EIA)
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Product Description
LS-F55421 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Ischemia Modified Albumin in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of Ischemia Modified Albumin as low as 157.2 nanograms per millilter.Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
Ischemia Modified Albumin
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Ischemia Modified Albumin (IMA). No significant cross-reactivity or interference between Ischemia Modified Albumin (IMA) and analogues was observed.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
370.4 - 30000 ng/ml
Sensitivity
157.2 ng/ml
Assay Time
2 h
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards.
- Add 50 uL standard or sample to each well.
- . . . .And then add 50 uL prepared Detection Reagent A immediately.
- . . . .Shake and mix.
- Incubate 1 hour at 37ºC.
- Aspirate and wash 3 times.
- Add 100 uL prepared Detection Reagent B.
- Incubate 30 minutes at 37ºC.
- Aspirate and wash 5 times.
- Add 90 uL Substrate Solution.
- Incubate 10-20 minutes at 37ºC.
- Add 50 uL Stop Solution.
- Read at 450 nm immediately.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Competition ELISA Platform Overview
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