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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 克隆性:
多克隆
- 抗体英文名:
Fluorescein Anti-Biotin (made in goat)
- 抗体名:
Fluorescein Anti-Biotin (made in goat)
- 规格:
0.5 mg
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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
( 一 ) 原理 目前用于抗体标记的荧光素主要有异硫氰酸荧光素 (Fluorescein isothiocynate,FITC) 或罗达明 (Lissamine rhodamine B200, RB200) 。在硷性条件下 FITC 的碳酰胺键可与抗体赖氨酸的ε氨基共价结合,标记后的抗体仍保持与相应抗原
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
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