- ￥12535
- Invivogen
- ant-zn-5
- 美国
- 2026年03月05日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- CAS号：
/
- 供应商：
欣博盛
- 库存：
大量
- 保质期：
见包装说明
- 英文名：
Zeocin™
- 保存条件：
Room temperature
- 规格：
5g (50 x 1 ml)
Zeocin™ (solution)/博莱霉素(InvivoGen专利产品)
品牌： Invivogen
产品货号： ant-zn-5
规格： 5g (50 x 1 ml)
Selection antibiotic for bacteria and mammalian cells – Endotoxin-tested
Zeocin® is a formulation of phleomycin D1 used for the selection of bacteria and eukaryotic cells, provided as a sterile, cell culture-tested solution or powder. This member of the bleomycin/phleomycin family is a water-soluble, copper-chelated glycopeptide antibiotic, produced by a proprietary strain of Streptomyces verticillus. Zeocin® causes cell death by intercalating into DNA and cleaving it. The action of Zeocin® is effective on most aerobic cells, such as bacteria, eukaryotic microorganisms, plant cells, and animal cells.
Resistance to Zeocin® is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin® upon binding to the antibiotic [1-3]. Zeocin® is an effective antibiotic for the selection of bacteria and eukaryotic cells expressing the Sh ble resistance.
Due to its activity in both E. coli and mammalian cell lines, vectors can be designed that carry only one drug resistance marker for selection. Suggested concentrations are 50 - 400 µg/ml for selection in mammalian cells and 25 - 50 µg/ml for selection in E. coli.
Key features
Produced in InvivoGen's facilities. InvivoGen is the sole producer of Zeocin®
Each lot thoroughly tested to ensure lot-to-lot reproducibility
Potency validated in Zeocin®-sensitive and Zeocin®-resistant mammalian cell lines
Absence of long-term effects confirmed in Zeocin®-resistant cells
Specifications
PRODUCT CONCENTRATION
100 mg/ml
APPLICATIONS
Eukaryotic selection/stable cell line generation (tested), bacterial selection (tested)
CAS NUMBER
11006-33-0
BUFFER
HEPES
PH
7.1 - 7.7
APPEARANCE (FORM)
Liquid
APPEARANCE (COLOR)
Blue
WORKING CONCENTRATION
50 - 400 µg/ml (mammalian cells), 25 - 50 µg/ml (E. coli)
STERILITY
0.2 µm filtration
ENDOTOXIN
< 1 EU/mg
QUALITY CONTROL
Each lot is functionally tested and validated.
Invivogen公司是一家基因治疗相关的分子生物学试剂公司，公司3212年在美国加州生物公司聚集地圣地亚哥成立，初期即以其拳头产品支原体抗生素与同城Invitrogen公司合作进行全球市场推广。其主打产品有：多基因表达质粒载体、DNA疫苗质粒载体、鼠类转基因质粒载体、合成基因质粒载体pMOD、腺病毒载体AdenoVec、基因转染试剂和一些非常具有特色的试剂盒，此外该公司还拥有很大的基因和启动子库，可以接受客户的定制服务，极大地方便了客户。
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- 作者
- 内容
- 询问日期
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7. Kanamori A. et al., 2002. Distinct sulfation requirements of selectins disclosed using cells that support rolling mediated by all three selectins under shear flow. L-selectin prefers carbohydrate 6-sulfation totyrosine sulfation, whereas p-selectin does not. J Biol Chem. 277(36):32578-86.
8. Ahmed et al., 2013. TRIF-mediated TLR3 and TLR4 signaling is negatively regulated by ADAM15. J Immunol. 190(5):2217-28.
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13. Kim HS. et al., 2004. Insulin-like growth factor-binding protein 3 induces caspase-dependent apoptosis through a death receptor-mediated pathway in MCF-7 human breast cancer cells. Cancer Res. 64(6):2229-37.
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15. Waak J. et al., 2009. Oxidizable residues mediating protein stability and cytoprotective interaction of DJ-1 with apoptosis signal-regulating kinase 1. J Biol Chem. 284(21):14245-57.
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