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Neobioscience
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文献和实验POLY-L-LYSINE COATED SLIDES SOLUTIONS --5% Chromic acid --0.01% Poly-L-Lysine (PLL) - made from a dry protein, with distilled water. (100mg/1 litre) This procedure can be carried out on either a ski or carousel staining
1 mg of total RNA dissolved in RNase-free water to 300 ul of the Oligotex-dT30 suspension. 2. Incubate for 3 minutes at 65 C. Chill on ice. 3. Add 0.2 volume of 5M NaCl. Incubate for 10 minutes at 37 C. 4. Centrifuge for 3 minutes
Silane and Poly-L-Lysine Coating of Microscope Slides
free environment for 5 minutes. Poly-L-Lysine Coating a) Smear 20ul of poly-l-lysine over each slide. b) Air dry in a dust free environment. Silated Slides a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma
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