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Neobioscience
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2 x 2.5 nmol
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文献和实验sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected a priori with computational methods. For example, primer dimer detection, amplification of alternative products, stem loop interference
Real-Time Primer Design for DNA Chips
base pair of a paired chain. It is assumed to be influenced by neighboring bases. More details can be seen in . Table 1. Nearest neighbor thermodynamics values GC content Chemically, hydrogen bonds between the bases of the primer and the gene
Optimal arbitrary primer length for Differential Display(图)
suggests that the optimal selectivity of the arbitrary primers should be provided by no more than 7 bases for each pair of primers to display 50-100 mRNAs.The minimum primer length for PCR is 9 bases.The arbitrary primers have to hybridize in a predictable
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