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- 供应商：
Neobioscience
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500 pouches
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文献和实验SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. Warm 2X RPMI + 20% FCS to 40 in waterbath. Allow at least 30 minutes for temperature
Hard Agar Medium for Egg Collection Plates
This is a firm medium that keeps well and makes a good base for egg collecting. Wrap filled petri dishes and store at 4-- 14o C (they should keep for at least 6 weeks). Substitute molasses for the corn syrup if you prefer
Hints and precautions for the care, feeding and breeding of Neurospora
Practices Stockkeeping Mutagenesis and enrichment Tips for encouraging colonial growth Solutions and media Handling Heterokaryons CROSSING 1. Temperature; glassware. Crosses are usually made at 25°C. Perithecia do not develop, or do so poorly
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